Anti-FAN1 antibody (801-851 aa) (STJ196098)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the human FAN1 at the amino acid range 801-851
General Information
| Short Description | Rabbit polyclonal anti-Fanconi-associated nuclease 1 (801-851 aa) for use in WB in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | FAN1 |
| Gene ID | 22909 |
| Uniprot ID | FAN1_HUMAN |
| Immunogen | Synthesized peptide derived from the human FAN1 at the amino acid range 801-851 |
| Immunogen Region | 801-851 aa |
| Specificity | This antibody detects endogenous levels of FAN1 at Human/Mouse |
Additional Info
| Function | Nuclease required for the repair of DNA interstrand cross-links (ICL) recruited at sites of DNA damage by monoubiquitinated FANCD2. Specifically involved in repair of ICL-induced DNA breaks by being required for efficient homologous recombination, probably in the resolution of homologous recombination intermediates. Not involved in DNA double-strand breaks resection. Acts as a 5'-3' exonuclease that anchors at a cut end of DNA and cleaves DNA successively at every third nucleotide, allowing to excise an ICL from one strand through flanking incisions. Probably keeps excising with 3'-flap annealing until it reaches and unhooks the ICL. Acts at sites that have a 5'-terminal phosphate anchor at a nick or a 1- or 2-nucleotide flap and is augmented by a 3' flap. Also has endonuclease activity toward 5'-flaps. |
| Protein Name | Fanconi-Associated Nuclease 1Fancd2/Fanci-Associated Nuclease 1Hfan1Myotubularin-Related Protein 15 |
| Database Links | Reactome: R-HSA-6783310 |
| Cellular Localisation | NucleusLocalizes At Sites Of Dna Damage Following Recruitment By Monoubiquitinated Fancd2Localizes To Stalled Replication Forks Via Its Ubz4-Type Zinc Finger |
| Alternative Antibody Names | Anti-Fanconi-Associated Nuclease 1 antibodyAnti-Fancd2/Fanci-Associated Nuclease 1 antibodyAnti-Hfan1 antibodyAnti-Myotubularin-Related Protein 15 antibodyAnti-FAN1 antibodyAnti-KIAA1018 antibodyAnti-MTMR15 antibody |
Information sourced from Uniprot.org