• Immunofluorescence analysis of L929 cells using FAM3D Rabbit polyclonal antibody (STJ119848) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of FAM3D in paraffin-embedded Human colon using FAM3D Rabbit polyclonal antibody (STJ119848) at dilution of 1:50 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of FAM3D in paraffin-embedded Rat ovary using FAM3D Rabbit polyclonal antibody (STJ119848) at dilution of 1:50 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of FAM3D in paraffin-embedded Mouse testis using FAM3D Rabbit polyclonal antibody (STJ119848) at dilution of 1:50 (20x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using FAM3D Rabbit polyclonal antibody (STJ119848) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-FAM3D antibody (24-204) (STJ119848)

SKU:
STJ119848

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-FAM3D (24-204) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FAM3D
Gene ID: 131177
Uniprot ID: FAM3D_HUMAN
Immunogen Region: 24-204
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 24-204 of human FAM3D (NP_620160.1).
Immunogen Sequence: RSYMSFSMKTIRLPRWLAAS PTKEIQVKKYKCGLIKPCPA NYFAFKICSGAANVVGPTMC FEDRMIMSPVKNNVGRGLNI ALVNGTTGAVLGQKAFDMYS GDVMHLVKFLKEIPGGALVL VASYDDPGTKMNDESRKLFS DLGSSYAKQLGFRDSWVFIG AKDLRGKSPFEQFLKNSPDT N
Tissue Specificity Abundantly expressed in placenta and weakly expressed in small intestine.
Protein Name Protein Fam3d
Cellular Localisation Secreted
Alternative Antibody Names Anti-Protein Fam3d antibody
Anti-FAM3D antibody
Anti-UNQ567 antibody
Anti-PRO1130 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance