| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | FAM32A |
| Gene ID: | 26017 |
| Uniprot ID: | FA32A_HUMAN |
| Immunogen Region: | 1-112 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-112 of human FAM32A (NP_054796.1). |
| Immunogen Sequence: | MEAYEQVQKGPLKLKGVAEL GVTKRKKKKKDKDKAKLLEA MGTSKKNEEEKRRGLDKRTP AQAAFEKMQEKRQMERILKK ASKTHKQRVEDFNRHLDTLT EHYDIPKVSWTK |
| Tissue Specificity | Expressed in ovary, with isoform 1 being predominant. |
| Function | Isoform 1, but not isoform 2 or isoform 3, may induce G2 arrest and apoptosis. May also increase cell sensitivity to apoptotic stimuli. |
| Protein Name | Protein Fam32aOvarian Tumor-Associated Gene 12Otag-12 |
| Database Links | Reactome: R-HSA-72163 |
| Cellular Localisation | Isoform 1: NucleusIsoform 2: Nucleus |
| Alternative Antibody Names | Anti-Protein Fam32a antibodyAnti-Ovarian Tumor-Associated Gene 12 antibodyAnti-Otag-12 antibodyAnti-FAM32A antibodyAnti-OTAG12 antibodyAnti-CGI-144 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and MRPL53 knockdown (KD) HeLa cells using [KD Validated] MRPL53 Rabbit polyclonal antibody (STJ11105622) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/178266/370594/STJ11105622_1__58411.1713136541.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and MRPL53 knockdown (KD) HeLa cells using [KD Validated] MRPL53 Rabbit polyclonal antibody (STJ11105622) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
