• Immunoprecipitation analysis of 600 Mu g extracts of Rat testis cells using 3 Mu g FAM160B2 antibody (STJ11101526). Western blot was performed from the immunoprecipitate using FAM160B2 antibody (STJ11101526) at a dilution of 1:1000.
  • Immunofluorescence analysis of NIH-3T3 cells using FAM160B2 Rabbit polyclonal antibody (STJ11101526) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of FAM160B2 in paraffin-embedded mouse kidney using FAM160B2 Rabbit polyclonal antibody (STJ11101526) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using FAM160B2 Rabbit polyclonal antibody (STJ11101526) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-FAM160B2 antibody (74-303) (STJ11101526)

SKU:
STJ11101526

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-FAM160B2 (74-303) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Fhip2b
Gene ID: 239170
Uniprot ID: FHI2B_MOUSE
Immunogen Region: 74-303
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 74-303 of mouse FAM160B2 (NP_919326.1).
Immunogen Sequence: GEAGPCLEYLLQHKILETLC TLGKAEYPPGMRQQVFQFFS KVLSQVQHPLLHYLSVHRPV QKLLRLGGTVPGSLTEKEEV QFTSVLCSKIQQDPELLAYI LEGKKIIGKKKTARESTAPP KDIAGYRDKDCPHSDALNRD PGLDKEHCGVPALSIHLPAE TEGPENGPGESNLITSLLGL CKSKKSRLALKAQENILLLV SVASPAAATYLTQSTSCCMA IAEHLCQLYR
Tissue Specificity Expressed in colon.
Function Able to activate MAPK/ERK and TGFB signaling pathways. May regulate the activity of genes involved in intestinal barrier function and immunoprotective inflammation. May play a role in cell proliferation.
Protein Name Fhf Complex Subunit Hook-Interacting Protein 2b
Retinoic Acid-Induced Protein 16
Alternative Antibody Names Anti-Fhf Complex Subunit Hook-Interacting Protein 2b antibody
Anti-Retinoic Acid-Induced Protein 16 antibody
Anti-Fhip2b antibody
Anti-Fam160b2 antibody
Anti-Rai16 antibody

Information sourced from Uniprot.org

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