• Confocal imaging of NIH/3T3 cells using FAAH1 Rabbit monoclonal antibody (STJ11101996, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Western blot analysis of various lysates using FAAH1 Rabbit monoclonal antibody (STJ11101996) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of various lysates using FAAH1 Rabbit monoclonal antibody (STJ11101996) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-FAAH antibody (480-579) [S6MR] (STJ11101996)

SKU:
STJ11101996

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-FAAH1 (480-579) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S6MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FAAH
Gene ID: 2166
Uniprot ID: FAAH1_HUMAN
Immunogen Region: 480-579
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 480-579 of human FAAH1 (O00519).
Immunogen Sequence: DLNAPGRATGAVSYTMLYNC LDFPAGVVPVTTVTAEDEAQ MEHYRGYFGDIWDKMLQKGM KKSVGLPVAVQCVALPWQEE LCLRFMREVERLMTPEKQSS
Tissue Specificity Highly expressed in the brain, small intestine, pancreas, skeletal muscle and testis. Also expressed in the kidney, liver, lung, placenta and prostate.
Function Catalyzes the hydrolysis of endogenous amidated lipids like the sleep-inducing lipid oleamide ((9Z)-octadecenamide), the endocannabinoid anandamide (N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-ethanolamine), as well as other fatty amides, to their corresponding fatty acids, thereby regulating the signaling functions of these molecules. Hydrolyzes polyunsaturated substrate anandamide preferentially as compared to monounsaturated substrates. It can also catalyze the hydrolysis of the endocannabinoid 2-arachidonoylglycerol (2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol). FAAH cooperates with PM20D1 in the hydrolysis of amino acid-conjugated fatty acids such as N-fatty acyl glycine and N-fatty acyl-L-serine, thereby acting as a physiological regulator of specific subsets of intracellular, but not of extracellular, N-fatty acyl amino acids.
Protein Name Fatty-Acid Amide Hydrolase 1
Anandamide Amidohydrolase 1
Fatty Acid Ester Hydrolase
Oleamide Hydrolase 1
Database Links Reactome: R-HSA-2142753
Cellular Localisation Endomembrane System
Single-Pass Membrane Protein
Cytoplasm
Cytoskeleton
Seems To Be Attached To Intracellular Membranes And A Portion Of The Cytoskeletal Network
Alternative Antibody Names Anti-Fatty-Acid Amide Hydrolase 1 antibody
Anti-Anandamide Amidohydrolase 1 antibody
Anti-Fatty Acid Ester Hydrolase antibody
Anti-Oleamide Hydrolase 1 antibody
Anti-FAAH antibody
Anti-FAAH1 antibody

Information sourced from Uniprot.org

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