• Western blot analysis of various lysates, using F12 rabbit polyclonal antibody (STJ23595) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunofluorescence analysis of HepG2 cells using F12 rabbit polyclonal antibody (STJ23595) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-F12 antibody (336-615) (STJ23595)

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STJ23595

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-F12 (336-615) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: F12
Gene ID: 2161
Uniprot ID: FA12_HUMAN
Immunogen Region: 336-615
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 336-615 of human F12 (NP_000496.2).
Immunogen Sequence: QTPGALPAKREQPPSLTRNG PLSCGQRLRKSLSSMTRVVG GLVALRGAHPYIAALYWGHS FCAGSLIAPCWVLTAAHCLQ DRPAPEDLTVVLGQERRNHS CEPCQTLAVRSYRLHEAFSP VSYQHDLALLRLQEDADGSC ALLSPYVQPVCLPSGAARPS ETTLCQVAGWGHQFEGAEEY ASFLQEAQVPFLSLERCSAP DVHGSSILPGMLCAGFLEGG TDACQGDSGGPLVCEDQAA
Post Translational Modifications Factor XII is activated by kallikrein in alpha-factor XIIa, which is further converted by trypsin into beta-factor XIIa. Alpha-factor XIIa is composed of an NH2-terminal heavy chain, called coagulation factor XIIa heavy chain, and a COOH-terminal light chain, called coagulation factor XIIa light chain, connected by a disulfide bond. Beta-factor XIIa is composed of 2 chains linked by a disulfide bond, an N-terminal nonapeptide, called beta-factor XIIa part 1, and coagulation factor XIIa light chain, also known in this context as beta-factor XIIa part 2. O- and N-glycosylated. The O-linked polysaccharides were not identified, but are probably the mucin type linked to GalNAc.
Function Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta-factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa.
Protein Name Coagulation Factor Xii
Hageman Factor
Haf Cleaved Into - Coagulation Factor Xiia Heavy Chain - Beta-Factor Xiia Part 1 - Coagulation Factor Xiia Light Chain
Beta-Factor Xiia Part 2
Database Links Reactome: R-HSA-140837
Reactome: R-HSA-9657688
Reactome: R-HSA-9657689
Cellular Localisation Secreted
Alternative Antibody Names Anti-Coagulation Factor Xii antibody
Anti-Hageman Factor antibody
Anti-Haf Cleaved Into - Coagulation Factor Xiia Heavy Chain - Beta-Factor Xiia Part 1 - Coagulation Factor Xiia Light Chain antibody
Anti-Beta-Factor Xiia Part 2 antibody
Anti-F12 antibody

Information sourced from Uniprot.org

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