• Human breast ductal carcinoma stained with anti-EZH2 antibody using peroxidase-conjugate and DAB chromogen. Note nuclear staining of carcinoma cells.

Anti-EZH2 antibody (C-Term) [ZR150] (STJ180568)

SKU:
STJ180568

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: IHC-P
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-EZH2 (C-Term) is suitable for use in Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: ZR150
Conjugation: Unconjugated
Isotype: IgG
Formulation: Tris-HCI buffer containing stabilizing protein (BSA) and <0.1% ProClin
Purification: Affinity purified
Dilution Range: 1:100-200
Storage Instruction: Store at 2‐8°C for up to 24 months. Predilute: Ready to use, no reconstitution necessary. Concentrate: Use dilution range and appropriate lab‐standardized diluent. Stability after dilution: 7 days at 24°C, 3 months at 2‐8°C, 6months at ‐20°C.
Gene Symbol: EZH2
Gene ID: 2146
Uniprot ID: EZH2_HUMAN
Immunogen Region: C-Term
Specificity: Positive Control: Breast carcinoma
Immunogen: Synthetic peptide corresponding to the C-terminus of human EZH2 protein
Tissue Specificity In the ovary, expressed in primordial follicles and oocytes and also in external follicle cells (at protein level). Expressed in many tissues. Overexpressed in numerous tumor types including carcinomas of the breast, colon, larynx, lymphoma and testis.
Post Translational Modifications Phosphorylated by AKT1. Phosphorylation by AKT1 reduces methyltransferase activity. Phosphorylation at Thr-345 by CDK1 and CDK2 promotes maintenance of H3K27me3 levels at EZH2-target loci, thus leading to epigenetic gene silencing. Sumoylated. Glycosylated: O-GlcNAcylation at Ser-75 by OGT increases stability of EZH2 and facilitates the formation of H3K27me3 by the PRC2/EED-EZH2 complex.
Function Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Displays a preference for substrates with less methylation, loses activity when progressively more methyl groups are incorporated into H3K27, H3K27me0 > H3K27me1 > H3K27me2. Compared to EZH1-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4 and the nuclear receptor RORA. Regulates the circadian clock via histone methylation at the promoter of the circadian genes. Essential for the CRY1/2-mediated repression of the transcriptional activation of PER1/2 by the CLOCK-BMAL1 heterodimer.involved in the di and trimethylation of 'Lys-27' of histone H3 on PER1/2 promoters which is necessary for the CRY1/2 proteins to inhibit transcription.
Protein Name Histone-Lysine N-Methyltransferase Ezh2
Enx-1
Enhancer Of Zeste Homolog 2
Lysine N-Methyltransferase 6
Database Links Reactome: R-HSA-212300
Reactome: R-HSA-2559580
Reactome: R-HSA-3214841
Reactome: R-HSA-5617472
Reactome: R-HSA-8943724
Reactome: R-HSA-8953750
Reactome: R-HSA-9609690
Reactome: R-HSA-9710421
Cellular Localisation Nucleus
Localizes To The Inactive X Chromosome In Trophoblast Stem Cells
Alternative Antibody Names Anti-Histone-Lysine N-Methyltransferase Ezh2 antibody
Anti-Enx-1 antibody
Anti-Enhancer Of Zeste Homolog 2 antibody
Anti-Lysine N-Methyltransferase 6 antibody
Anti-EZH2 antibody
Anti-KMT6 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance