Anti-EZH2 antibody (C-Term) [ZR150] (STJ180568)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthetic peptide corresponding to the C-terminus of human EZH2 protein
General Information
| Short Description | Rabbit monoclonal EZH2 (C-Term) antibody for use in IHC-P in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC-P |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | ZR150 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity purified |
| Dilution Range | 1:100-200 |
| Formulation | Tris-HCI buffer containing stabilizing protein (BSA) and <0.1% ProClin |
| Storage Instruction | Store at 2β8Β°C for up to 24 months. Predilute: Ready to use, no reconstitution necessary. Concentrate: Use dilution range and appropriate labβstandardized diluent. Stability after dilution: 7 days at 24Β°C, 3 months at 2β8Β°C, 6months at β20Β°C. |
Target Information
| Gene Symbol | EZH2 |
| Gene ID | 2146 |
| Uniprot ID | EZH2_HUMAN |
| Immunogen | Synthetic peptide corresponding to the C-terminus of human EZH2 protein |
| Immunogen Region | C-Term |
| Specificity | Positive Control: Breast carcinoma |
Additional Info
| Tissue Specificity | In the ovary, expressed in primordial follicles and oocytes and also in external follicle cells (at protein level). Expressed in many tissues. Overexpressed in numerous tumor types including carcinomas of the breast, colon, larynx, lymphoma and testis. |
| Post Translational Modifications | Phosphorylated by AKT1. Phosphorylation by AKT1 reduces methyltransferase activity. Phosphorylation at Thr-345 by CDK1 and CDK2 promotes maintenance of H3K27me3 levels at EZH2-target loci, thus leading to epigenetic gene silencing. Sumoylated. Glycosylated: O-GlcNAcylation at Ser-75 by OGT increases stability of EZH2 and facilitates the formation of H3K27me3 by the PRC2/EED-EZH2 complex. |
| Function | Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Displays a preference for substrates with less methylation, loses activity when progressively more methyl groups are incorporated into H3K27, H3K27me0 > H3K27me1 > H3K27me2. Compared to EZH1-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4 and the nuclear receptor RORA. Regulates the circadian clock via histone methylation at the promoter of the circadian genes. Essential for the CRY1/2-mediated repression of the transcriptional activation of PER1/2 by the CLOCK-BMAL1 heterodimer.involved in the di and trimethylation of 'Lys-27' of histone H3 on PER1/2 promoters which is necessary for the CRY1/2 proteins to inhibit transcription. |
| Protein Name | Histone-Lysine N-Methyltransferase Ezh2Enx-1Enhancer Of Zeste Homolog 2Lysine N-Methyltransferase 6 |
| Database Links | Reactome: R-HSA-212300Reactome: R-HSA-2559580Reactome: R-HSA-3214841Reactome: R-HSA-5617472Reactome: R-HSA-8943724Reactome: R-HSA-8953750Reactome: R-HSA-9609690Reactome: R-HSA-9710421 |
| Cellular Localisation | NucleusLocalizes To The Inactive X Chromosome In Trophoblast Stem Cells |
| Alternative Antibody Names | Anti-Histone-Lysine N-Methyltransferase Ezh2 antibodyAnti-Enx-1 antibodyAnti-Enhancer Of Zeste Homolog 2 antibodyAnti-Lysine N-Methyltransferase 6 antibodyAnti-EZH2 antibodyAnti-KMT6 antibody |
Information sourced from Uniprot.org