• Western blot analysis of extracts of various cell lines, using EXT1 antibody (STJ23589) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST.
  • Immunofluorescence analysis of HeLa cells using EXT1 rabbit polyclonal antibody (STJ23589) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using EXT1 rabbit polyclonal antibody (STJ23589) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using EXT1 rabbit polyclonal antibody (STJ23589) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using EXT1 rabbit polyclonal antibody (STJ23589) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-EXT1 antibody (467-746) (STJ23589)

SKU:
STJ23589

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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EXT1
Gene ID: 2131
Uniprot ID: EXT1_HUMAN
Immunogen Region: 467-746
Specificity: A synthetic peptide corresponding to a sequence within amino acids 467-746 of human EXT1 (NP_000118.2).
Immunogen Sequence: YYYANLGLKPPSKFTAVIHA VTPLVSQSQPVLKLLVAAAK SQYCAQIIVLWNCDKPLPAK HRWPATAVPVVVIEGESKVM SSRFLPYDNIITDAVLSLDE DTVLSTTEVDFAFTVWQSFP ERIVGYPARSHFWDNSKERW GYTSKWTNDYSMVLTGAAIY HKYYHYLYSHYLPASLKNMV DQLANCEDILMNFLVSAVTK LPPIKVTQKKQYKETMMGQT SRASRWADPDHFAQRQSCM
Tissue Specificity Widely expressed.
Post Translational Modifications N-glycosylated.
Function Glycosyltransferase forming with EXT2 the heterodimeric heparan sulfate polymerase which catalyzes the elongation of the heparan sulfate glycan backbone. Glycan backbone extension consists in the alternating transfer of (1->4)-beta-D-GlcA and (1->4)-alpha-D-GlcNAc residues from their respective UDP-sugar donors. Both EXT1 and EXT2 are required for the full activity of the polymerase since EXT1 bears the N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase activity within the complex while EXT2 carries the glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase activity. Heparan sulfate proteoglycans are ubiquitous components of the extracellular matrix and play an important role in tissue homeostasis and signaling.
Protein Name Exostosin-1
Exostosin Glycosyltransferase 1
Heparan Sulfate Co-Polymerase Subunit Ext1
Multiple Exostoses Protein 1
N-Acetylglucosaminyl-Proteoglycan 4-Beta-Glucuronosyltransferase
Database Links Reactome: R-HSA-2022928
Reactome: R-HSA-3656237
Reactome: R-HSA-3656253
Cellular Localisation Golgi Apparatus Membrane
Single-Pass Type Ii Membrane Protein
Golgi Apparatus
Cis-Golgi Network Membrane
Endoplasmic Reticulum Membrane
The Active Heparan Sulfate Polymerase Complex Composed Of Ext1 And Ext2 Is Localized To The Golgi Apparatus
If Both Proteins Are Individually Detected In The Endoplasmic Reticulum
The Formation Of The Complex Promotes Their Transport To The Golgi
Alternative Antibody Names Anti-Exostosin-1 antibody
Anti-Exostosin Glycosyltransferase 1 antibody
Anti-Heparan Sulfate Co-Polymerase Subunit Ext1 antibody
Anti-Multiple Exostoses Protein 1 antibody
Anti-N-Acetylglucosaminyl-Proteoglycan 4-Beta-Glucuronosyltransferase antibody
Anti-EXT1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance