Anti-EWSR1 antibody (1-189) [S3MR] (STJ11102513)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal EWSR1 (1-189) antibody for use in WB, IHC-P, IF, ICC, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S3MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specif |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | EWSR1 |
| Gene ID | 2130 |
| Uniprot ID | EWS_HUMAN |
| Immunogen Region | 1-189 |
| Immunogen Sequence | MASTDYSTYSQAAAQQGYSA YTAQPTQGYAQTTQAYGQQS YGTYGQPTDVSYTQAQTTAT YGQTAYATSYGQPPTGYTTP TAPQAYSQPVQGYGTGAYDT TTATVTTTQASYAAQSAYGT QPAYPAYGQQPAATAPTRPQ DGNKPTETSQPQSSTGGYNQ PSLGYGQSNYSYPQVPGSYP MQPVTAPPS |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-189 of human EWSR1 (Q01844). |
Additional Info
| Tissue Specificity | Ubiquitous. |
| Post Translational Modifications | Phosphorylated.calmodulin-binding inhibits phosphorylation of Ser-266. Highly methylated on arginine residues. Methylation is mediated by PRMT1 and, at lower level by PRMT8. |
| Function | Binds to ssRNA containing the consensus sequence 5'-AGGUAA-3'. Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes. |
| Protein Name | Rna-Binding Protein EwsEws OncogeneEwing Sarcoma Breakpoint Region 1 Protein |
| Database Links | |
| Cellular Localisation | NucleusCytoplasmCell MembraneRelocates From Cytoplasm To Ribosomes Upon Ptk2b/Fak2 Activation |
| Alternative Antibody Names | Anti-Rna-Binding Protein Ews antibodyAnti-Ews Oncogene antibodyAnti-Ewing Sarcoma Breakpoint Region 1 Protein antibodyAnti-EWSR1 antibodyAnti-EWS antibody |
Information sourced from Uniprot.org