• Western blot analysis of lysates from HeLa cells, using EV71 3D Rabbit polyclonal antibody (STJ11103415) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-EV71 3D antibody (1-217 aa) (STJ11103415)

SKU:
STJ11103415

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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 1-217 aa
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-217 of Enterovirus EV71 3D (B9VUU3).
Immunogen Sequence: MGSQVSTQRSGSHENSNSAT EGSTINYTTINYYKDSYAAT AGKQSLKQDPDKFANPVKDI FTEMAAPLKSPSAEACGYSD RVAQLTIGNSTITTQEAANI IVGYGEWPSYCSDSDATAVD KPTRPDVSVNRFYTLDTKLW EKSSKGWYWKFPDVLTETGV FGQNAQFHYLYRSGFCIHVQ CNASKFHQGALLVAVLPEYV IGTVAGGTGTEDSHPPY
Background Enterovirus A 71 (EV71) is a neurotropic virus that may lead to acute flaccid paralysis, encephalitis, cardiopulmonary failure or even death. No vaccine and defensive drug controlling EV71 is currently available, novel and efficient antiviral drug or vaccine is therefore urgently needed. 3Dpol (RNA-dependent RNA polymerase (RdRp) ) has been an important target for anti-EV71 drug development.

Information sourced from Uniprot.org

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