Anti-Eukaryotic translation initiation factor 2C antibody (C-Term) (STJA0040935)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide corresponding to a sequence (845-860 aa) from the C-terminus of mouse elF2C conjugated to KLH.
General Information
| Short Description | Rabbit polyclonal anti-Eukaryotic translation initiation factor 2C (C-Term) for use in IHC-F, IHC-P, IP and WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC-F/IHC-P/IP/WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity-purified |
| Dilution Range | WB 1:1000-1:2000IHC 1:200-1:500 |
| Formulation | Lyophilized from PBS. |
| Storage Instruction | Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at |
Target Information
| Gene Symbol | Ago2 |
| Gene ID | 239528 |
| Uniprot ID | AGO2_MOUSE |
| Immunogen | A synthetic peptide corresponding to a sequence (845-860 aa) from the C-terminus of mouse elF2C conjugated to KLH. |
| Immunogen Region | C-Term |
| Immunogen Sequence | Mouse |
| Specificity | Specificity has been confirmed by IHC, WB and IP in mouse brain. This antibody is expected to recognize at least elF2c-1, 2 and 3 since they have identical C-termini. Human; mouse; rat; The Ago proteins are highly conserved so cross-reactivity with o |
Additional Info
| Tissue Specificity | Ubiquitous expression in 9.5 day embryos with highest levels in forebrain, heart, limb buds, and branchial arches. |
| Post Translational Modifications | Hydroxylated. 4-hydroxylation appears to enhance protein stability but is not required for miRNA-binding or endonuclease activity. Ubiquitinated on surface-exposed lysines by a SCF-like E3 ubiquitin-protein ligase complex containing ZSWIM8 during target-directed microRNA degradation (TDMD), a process that mediates degradation of microRNAs (miRNAs). Ubiquitination by the SCF-like E3 ubiquitin-protein ligase complex containing ZSWIM8 leads to its subsequent degradation, thereby exposing miRNAs for degradation. ZSWIM8 recognizes and binds AGO2 when it is engaged with a TDMD target. Phosphorylation at Ser-388 by AKT3.leads to up-regulate translational repression of microRNA target and down-regulate endonucleolytic cleavage. A phosphorylation cycle of C-terminal serine cluster (Ser-825-Ser-835) regulates the release of target mRNAs. Target-binding leads to phosphorylation of these residues by CSNK1A1, which reduces the affinity of AGO2 for mRNA and enables target release. The ANKRD52-PPP6C phosphatase complex dephosphorylates the residues, which primes AGO2 for binding a new target. |
| Function | Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). Can also up-regulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3'-UTR of the TNF (TNF-alpha) mRNA and up-regulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions. Regulates lymphoid and erythroid development and function, and this is independent of endonuclease activity. |
| Protein Name | Protein Argonaute-2Argonaute2Mago2Argonaute Risc Catalytic Component 2Eukaryotic Translation Initiation Factor 2c 2Eif-2c 2Eif2c 2Piwi/Argonaute Family Protein Meif2c2Protein Slicer |
| Database Links | Reactome: R-MMU-203927Reactome: -MMU-426486Reactome: -MMU-426496Reactome: -MMU-5578749 |
| Cellular Localisation | CytoplasmP-BodyNucleusTranslational Repression Of Mrnas Results In Their Recruitment To P-BodiesTranslocation To The Nucleus Requires Imp8 |
| Alternative Antibody Names | Anti-Protein Argonaute-2 antibodyAnti-Argonaute2 antibodyAnti-Mago2 antibodyAnti-Argonaute Risc Catalytic Component 2 antibodyAnti-Eukaryotic Translation Initiation Factor 2c 2 antibodyAnti-Eif-2c 2 antibodyAnti-Eif2c 2 antibodyAnti-Piwi/Argonaute Family Protein Meif2c2 antibodyAnti-Protein Slicer antibodyAnti-Ago2 antibodyAnti-Eif2c2 antibodyAnti-Kiaa4215 antibody |
Information sourced from Uniprot.org