• Immunofluorescence analysis of U-2 OS cells using Pea3/ ETV4 Rabbit polyclonal antibody (STJ28361) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Pea3/ ETV4 in paraffin-embedded Rat skin using Pea3/ ETV4 Rabbit polyclonal antibody (STJ28361) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Pea3/ ETV4 in paraffin-embedded Mouse skin using Pea3/ ETV4 Rabbit polyclonal antibody (STJ28361) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Pea3/ ETV4 in paraffin-embedded Human skin using Pea3/ ETV4 Rabbit polyclonal antibody (STJ28361) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using Pea3/ ETV4 Rabbit polyclonal antibody (STJ28361) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of various lysates using Pea3/ ETV4 Rabbit polyclonal antibody (STJ28361) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-ETV4 antibody (40-246) (STJ28361)

SKU:
STJ28361

£112.50 - £522.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Pea3/ETV4 (40-246) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ETV4
Gene ID: 2118
Uniprot ID: ETV4_HUMAN
Immunogen Region: 40-246
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 40-246 of human ETV4 (NP_001977.1).
Immunogen Sequence: MDPGSLPPLDSEDLFQDLSH FQETWLAEAQVPDSDEQFVP DFHSENLAFHSPTTRIKKEP QSPRTDPALSCSRKPPLPYH HGEQCLYSSAYDPPRQIAIK SPAPGALGQSPLQPFPRAEQ RNFLRSSGTSQPHPGHGYLG EHSSVFQQPLDICHSFTSQG GGREPLPAPYQHQLSEPCPP YPQQSFKQEYHDPLYEQAGQ PAVDQGG
Tissue Specificity Expressed in keratinocytes.
Post Translational Modifications Sumoylated.enhanced upon ERK/MAP kinase pathway activation, it positively regulates the transcriptional activator capacity. Sumoylation at Lys-96 probably requires phosphorylation at Ser-101. Transiently polysumoylated and desumoylated by SENP1. Sumoylation is a prerequisite to polyubiquitination which in turn increases proteasomal-mediated degradation. Probably polyubiquitinated by RNF4 and deubiquitinated by USP2.
Function Transcriptional activator. May play a role in keratinocyte differentiation. (Microbial infection) Binds to the enhancer of the adenovirus E1A gene and acts as a transcriptional activator.the core-binding sequence is 5'-ACGGAATGT-3'.
Protein Name Ets Translocation Variant 4
Adenovirus E1a Enhancer-Binding Protein
E1a-F
Polyomavirus Enhancer Activator 3 Homolog
Protein Pea3
Database Links Reactome: R-HSA-5687128
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Ets Translocation Variant 4 antibody
Anti-Adenovirus E1a Enhancer-Binding Protein antibody
Anti-E1a-F antibody
Anti-Polyomavirus Enhancer Activator 3 Homolog antibody
Anti-Protein Pea3 antibody
Anti-ETV4 antibody
Anti-E1AF antibody
Anti-PEA3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance