Western blot analysis of various lysates using ETFDH Rabbit polyclonal antibody (STJ28668) at 1:3000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime:ย 15s.

Anti-ETFDH antibody (318-617) (STJ28668)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ28668
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General Information

Short DescriptionRabbit polyclonal ETFDH (318-617) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:1000-1:5000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolETFDH
Gene ID2110
Uniprot IDETFD_HUMAN
Immunogen Region318-617
Immunogen SequenceLVALGLVVGLDYQNPYLSPF REFQRWKHHPSIRPTLEGGK RIAYGARALNEGGFQSIPKL TFPGGLLIGCSPGFMNVPKI KGTHTAMKSGILAAESIFNQ LTSENLQSKTIGLHVTEYED NLKNSWVWKELYSVRNIRPS CHGVLGVYGGMIYTGIFYWI LRGMEPWTLKHKGSDFERLK PAKDCTPIEYPKPDGQISFD LLSSVALSGTNHEHDQPAHL TLRDDSIPVNRNLSIYDGP
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 318-617 of human ETFDH (NP_004444.2).

Additional Info

Function Accepts electrons from ETF and reduces ubiquinone.
Protein Name Electron Transfer Flavoprotein-Ubiquinone Oxidoreductase - Mitochondrial
Etf-Qo
Etf-Ubiquinone Oxidoreductase
Electron-Transferring-Flavoprotein Dehydrogenase
Etf Dehydrogenase
Database Links Reactome: R-HSA-611105
Cellular Localisation Mitochondrion Inner Membrane
Alternative Antibody Names Anti-Electron Transfer Flavoprotein-Ubiquinone Oxidoreductase - Mitochondrial antibody
Anti-Etf-Qo antibody
Anti-Etf-Ubiquinone Oxidoreductase antibody
Anti-Electron-Transferring-Flavoprotein Dehydrogenase antibody
Anti-Etf Dehydrogenase antibody
Anti-ETFDH antibody

Information sourced from Uniprot.org

Citations

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