Host: | Goat |
Applications: | Pep-ELISA/WB/IHC |
Reactivity: | Human/Mouse/Rat/Pig/Dog/Cow |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description : | Goat polyclonal antibody anti-ETFA (139-152) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
Clonality : | Polyclonal |
Conjugation: | Unconjugated |
Isotype: | IgG |
Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Concentration: | 0.5 mg/mL |
Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 30+35kDa bands observed in Human Colon lysates (calculated MW of 35.1kDa according to NP_000117.1 and of 30.0kDa according to NP_001121188.1. Recommended concentration: 0.1-0.3µ |
Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Gene Symbol: | ETFA |
Gene ID: | 2108 |
Uniprot ID: | ETFA_HUMAN |
Immunogen Region: | 139-152 |
Accession Number: | NP_000117.1; NP_001121188.1 |
Specificity: | This antibody is expected to recognize both isoforms (NP_000117.1; NP_001121188.1). Amino acid numbering in name refers to NP_000117.1 sequence) |
Immunogen Sequence: | KSPDTFVRTIYAGN |
Function | Heterodimeric electron transfer flavoprotein that accepts electrons from several mitochondrial dehydrogenases, including acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). Required for normal mitochondrial fatty acid oxidation and normal amino acid metabolism. |
Protein Name | Electron Transfer Flavoprotein Subunit Alpha - MitochondrialAlpha-Etf |
Database Links | Reactome: R-HSA-611105 |
Cellular Localisation | Mitochondrion Matrix |
Alternative Antibody Names | Anti-Electron Transfer Flavoprotein Subunit Alpha - Mitochondrial antibodyAnti-Alpha-Etf antibodyAnti-ETFA antibody |
Information sourced from Uniprot.org