• Western blot analysis of various lysates using ETFA rabbit polyclonal antibody (STJ29983) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % non-fat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 1s.
  • Western blot analysis of various lysates using ETFA rabbit polyclonal antibody (STJ29983) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % non-fat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 10s.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using ETFA rabbit polyclonal antibody (STJ29983) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using ETFA rabbit polyclonal antibody (STJ29983) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver cancer using ETFA rabbit polyclonal antibody (STJ29983) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using ETFA rabbit polyclonal antibody (STJ29983) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using ETFA rabbit polyclonal antibody (STJ29983) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse spleen using ETFA rabbit polyclonal antibody (STJ29983) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunoprecipitation analysis of 300 Mu g extracts of 293T cells using 3 Mu g ETFA antibody (STJ29983). Western blot was performed from the immunoprecipitate using ETFA antibody (STJ29983) at a dilution of 1:1000.

Anti-ETFA antibody (1-333) (STJ29983)

SKU:
STJ29983

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ETFA (1-333) is suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ETFA
Gene ID: 2108
Uniprot ID: ETFA_HUMAN
Immunogen Region: 1-333
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-333 of human ETFA (NP_000117.1).
Immunogen Sequence: MFRAAAPGQLRRAASLLRFQ STLVIAEHANDSLAPITLNT ITAATRLGGEVSCLVAGTKC DKVAQDLCKVAGIAKVLVAQ HDVYKGLLPEELTPLILATQ KQFNYTHICAGASAFGKNLL PRVAAKLEVAPISDIIAIKS PDTFVRTIYAGNALCTVKCD EKVKVFSVRGTSFDAAATSG GSASSEKASSTSPVEISEWL DQKLTKSDRPELTGAKVVVS GGRGLKSGENFKLLYDLAD
Post Translational Modifications The N-terminus is blocked.
Function Heterodimeric electron transfer flavoprotein that accepts electrons from several mitochondrial dehydrogenases, including acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). Required for normal mitochondrial fatty acid oxidation and normal amino acid metabolism.
Protein Name Electron Transfer Flavoprotein Subunit Alpha - Mitochondrial
Alpha-Etf
Database Links Reactome: R-HSA-611105
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Electron Transfer Flavoprotein Subunit Alpha - Mitochondrial antibody
Anti-Alpha-Etf antibody
Anti-ETFA antibody

Information sourced from Uniprot.org

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