![Western blot analysis of lysates from wild type (WT) and ETFA knockout (KO) 293T cells, using [KO Validated] ETFA Rabbit polyclonal antibody (STJ11100813) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/89749/358226/STJ11100813_1__44625.1713122552.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and ETFA knockout (KO) 293T cells, using [KO Validated] ETFA Rabbit polyclonal antibody (STJ11100813) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western blot analysis of lysates from wild type (WT) and ETFA knockout (KO) 293T cells, using [KO Validated] ETFA Rabbit polyclonal antibody (STJ11100813) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/89749/358226/STJ11100813_1__44625.1713122552.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and ETFA knockout (KO) 293T cells, using [KO Validated] ETFA Rabbit polyclonal antibody (STJ11100813) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000IF/ICC:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | ETFA |
| Gene ID: | 2108 |
| Uniprot ID: | ETFA_HUMAN |
| Immunogen Region: | 1-333 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-333 of human ETFA (NP_000117.1). |
| Immunogen Sequence: | MFRAAAPGQLRRAASLLRFQ STLVIAEHANDSLAPITLNT ITAATRLGGEVSCLVAGTKC DKVAQDLCKVAGIAKVLVAQ HDVYKGLLPEELTPLILATQ KQFNYTHICAGASAFGKNLL PRVAAKLEVAPISDIIAIKS PDTFVRTIYAGNALCTVKCD EKVKVFSVRGTSFDAAATSG GSASSEKASSTSPVEISEWL DQKLTKSDRPELTGAKVVVS GGRGLKSGENFKLLYDLAD |
| Post Translational Modifications | The N-terminus is blocked. |
| Function | Heterodimeric electron transfer flavoprotein that accepts electrons from several mitochondrial dehydrogenases, including acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). Required for normal mitochondrial fatty acid oxidation and normal amino acid metabolism. |
| Protein Name | Electron Transfer Flavoprotein Subunit Alpha - MitochondrialAlpha-Etf |
| Database Links | Reactome: R-HSA-611105 |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative Antibody Names | Anti-Electron Transfer Flavoprotein Subunit Alpha - Mitochondrial antibodyAnti-Alpha-Etf antibodyAnti-ETFA antibody |
Information sourced from Uniprot.org