| Host: | Rabbit |
| Applications: | WB |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Rabbit polyclonal antibody anti-ERCC3 (512-782) is suitable for use in Western Blot research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: | Affinity purification |
| Dilution Range: | WB 1:500-1:2000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | ERCC3 |
| Gene ID: | 2071 |
| Uniprot ID: | ERCC3_HUMAN |
| Immunogen Region: | 512-782 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 512-782 of human ERCC3 (NP_000113.1). |
| Immunogen Sequence: | CPMSPEFYREYVAIKTKKRI LLYTMNPNKFRACQFLIKFH ERRNDKIIVFADNVFALKEY AIRLNKPYIYGPTSQGERMQ ILQNFKHNPKINTIFISKVG DTSFDLPEANVLIQISSHGG SRRQEAQRLGRVLRAKKGMV AEEYNAFFYSLVSQDTQEMA YSTKRQRFLVDQGYSFKVIT KLAGMEEEDLAFSTKEEQQQ LLQKVLAATDLDAEEEVVAG EFGSRSSQASRRFGTMSSM |
| Function | ATP-dependent 3'-5' DNA helicase, component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The ATPase activity of XPB/ERCC3, but not its helicase activity, is required for DNA opening. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. The ATP-dependent helicase activity of XPB/ERCC3 is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription. |
| Protein Name | General Transcription And Dna Repair Factor Iih Helicase Subunit XpbTfiih Subunit XpbBasic Transcription Factor 2 89 Kda SubunitBtf2 P89Dna Excision Repair Protein Ercc-3Dna Repair Protein Complementing Xp-B CellsTfiih Basal Transcription Factor Complex 89 Kda SubunitTfiih 89 Kda SubunitTfiih P89Xeroderma Pigmentosum Group B-Complementing Protein |
| Database Links | Reactome: R-HSA-112382Reactome: R-HSA-113418Reactome: R-HSA-167152Reactome: R-HSA-167158Reactome: R-HSA-167160Reactome: R-HSA-167161Reactome: R-HSA-167162Reactome: R-HSA-167172Reactome: R-HSA-167200Reactome: R-HSA-167246Reactome: R-HSA-427413Reactome: R-HSA-5696395Reactome: R-HSA-5696400Reactome: R-HSA-674695Reactome: R-HSA-6781823Reactome: R-HSA-6781827Reactome: R-HSA-6782135Reactome: R-HSA-6782210Reactome: R-HSA-6796648Reactome: R-HSA-72086Reactome: R-HSA-73762Reactome: R-HSA-73772Reactome: R-HSA-73776Reactome: R-HSA-73779Reactome: R-HSA-73863Reactome: R-HSA-75953Reactome: R-HSA-75955Reactome: R-HSA-76042Reactome: R-HSA-77075 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-General Transcription And Dna Repair Factor Iih Helicase Subunit Xpb antibodyAnti-Tfiih Subunit Xpb antibodyAnti-Basic Transcription Factor 2 89 Kda Subunit antibodyAnti-Btf2 P89 antibodyAnti-Dna Excision Repair Protein Ercc-3 antibodyAnti-Dna Repair Protein Complementing Xp-B Cells antibodyAnti-Tfiih Basal Transcription Factor Complex 89 Kda Subunit antibodyAnti-Tfiih 89 Kda Subunit antibodyAnti-Tfiih P89 antibodyAnti-Xeroderma Pigmentosum Group B-Complementing Protein antibodyAnti-ERCC3 antibodyAnti-XPB antibodyAnti-XPBC antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
