| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ERCC3 |
| Gene ID: |
2071 |
| Uniprot ID: |
ERCC3_HUMAN |
| Immunogen Region: |
512-782 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 512-782 of human ERCC3 (NP_000113.1). |
| Immunogen Sequence: |
CPMSPEFYREYVAIKTKKRI LLYTMNPNKFRACQFLIKFH ERRNDKIIVFADNVFALKEY AIRLNKPYIYGPTSQGERMQ ILQNFKHNPKINTIFISKVG DTSFDLPEANVLIQISSHGG SRRQEAQRLGRVLRAKKGMV AEEYNAFFYSLVSQDTQEMA YSTKRQRFLVDQGYSFKVIT KLAGMEEEDLAFSTKEEQQQ LLQKVLAATDLDAEEEVVAG EFGSRSSQASRRFGTMSSM |
| Post Translational Modifications | Phosphorylation on Ser-751 by CK2 controls the 5'-excision activity of ERCC1-XPF endonuclease.phosphorylated protein inhibits the excision activity and thus NER. Dephosphorylation reactivates the 5'-excision step. Phosphorylation has no effect on transcription or the 3'-5' helicase activity. |
| Function | ATP-dependent 3'-5' DNA helicase/translocase. Binds dsDNA rather than ssDNA, unzipping it in a translocase rather than classical helicase activity. Component of the general transcription and DNA repair factor IIH (TFIIH) core complex. When complexed to CDK-activating kinase (CAK), involved in RNA transcription by RNA polymerase II. The ATPase activity of XPB/ERCC3, but not its helicase activity, is required for DNA opening.it may wrap around the damaged DNA wedging it open, causing localized melting that allows XPD/ERCC2 helicase to anchor. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. The ATP-dependent helicase activity of XPB/ERCC3 is required for promoter opening and promoter escape. In transcription pre-initiation complexes induces and propagates a DNA twist to open DNA. Also involved in transcription-coupled nucleotide excision repair (NER) of damaged DNA. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The structure of the TFIIH transcription complex differs from the NER-TFIIH complex.large movements by XPD/ERCC2 and XPB/ERCC3 are stabilized by XPA. XPA retains XPB/ERCC3 at the 5' end of a DNA bubble (mimicking DNA damage). |
| Protein Name | General Transcription And Dna Repair Factor Iih Helicase/Translocase Subunit XpbTfiih Subunit XpbBasic Transcription Factor 2 89 Kda SubunitBtf2 P89Dna 3'-5' Helicase/Translocase XpbDna Excision Repair Protein Ercc-3Dna Repair Protein Complementing Xp-B CellsTfiih Basal Transcription Factor Complex 89 Kda SubunitTfiih 89 Kda SubunitTfiih P89Xeroderma Pigmentosum Group B-Complementing Protein |
| Database Links | Reactome: R-HSA-112382Reactome: R-HSA-113418Reactome: R-HSA-167152Reactome: R-HSA-167158Reactome: R-HSA-167160Reactome: R-HSA-167161Reactome: R-HSA-167162Reactome: R-HSA-167172Reactome: R-HSA-167200Reactome: R-HSA-167246Reactome: R-HSA-427413Reactome: R-HSA-5696395Reactome: R-HSA-5696400Reactome: R-HSA-674695Reactome: R-HSA-6781823Reactome: R-HSA-6781827Reactome: R-HSA-6782135Reactome: R-HSA-6782210Reactome: R-HSA-6796648Reactome: R-HSA-72086Reactome: R-HSA-73762Reactome: R-HSA-73772Reactome: R-HSA-73776Reactome: R-HSA-73779Reactome: R-HSA-73863Reactome: R-HSA-75953Reactome: R-HSA-75955Reactome: R-HSA-76042Reactome: R-HSA-77075 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-General Transcription And Dna Repair Factor Iih Helicase/Translocase Subunit Xpb antibodyAnti-Tfiih Subunit Xpb antibodyAnti-Basic Transcription Factor 2 89 Kda Subunit antibodyAnti-Btf2 P89 antibodyAnti-Dna 3'-5' Helicase/Translocase Xpb antibodyAnti-Dna Excision Repair Protein Ercc-3 antibodyAnti-Dna Repair Protein Complementing Xp-B Cells antibodyAnti-Tfiih Basal Transcription Factor Complex 89 Kda Subunit antibodyAnti-Tfiih 89 Kda Subunit antibodyAnti-Tfiih P89 antibodyAnti-Xeroderma Pigmentosum Group B-Complementing Protein antibodyAnti-ERCC3 antibodyAnti-XPB antibodyAnti-XPBC antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
