| Host: |
Rabbit |
| Applications: |
WB/IF/ICC/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
EPOR |
| Gene ID: |
2057 |
| Uniprot ID: |
EPOR_HUMAN |
| Immunogen Region: |
274-508 |
| Specificity: |
A synthetic peptide corresponding to a sequence within amino acids 274-508 of human EPOR (NP_000112.1). |
| Immunogen Sequence: |
HRRALKQKIWPGIPSPESEF EGLFTTHKGNFQLWLYQNDG CLWWSPCTPFTEDPPASLEV LSERCWGTMQAVEPGTDDEG PLLEPVGSEHAQDTYLVLDK WLLPRNPPSEDLPGPGGSVD IVAMDEGSEASSCSSALASK PSPEGASAASFEYTILDPSS QLLRPWTLCPELPPTPPHLK YLYLVVSDSGISTDYSSGDS QGAQGGLSDGPYSNPYENSL IPAAEPLPPSYVACS |
| Tissue Specificity | Erythroid cells and erythroid progenitor cells. Isoform EPOR-F: Isoform EPOR-F is the most abundant form in EPO-dependent erythroleukemia cells and in late-stage erythroid progenitors. Isoform EPOR-S: Isoform EPOR-S and isoform EPOR-T are the predominant forms in bone marrow. Isoform EPOR-T: Isoform EPOR-S and isoform EPOR-T are the predominant forms in bone marrow. Isoform EPOR-T is the most abundant from in early-stage erythroid progenitor cells. |
| Post Translational Modifications | On EPO stimulation, phosphorylated on C-terminal tyrosine residues by JAK2. The phosphotyrosine motifs are also recruitment sites for several SH2-containing proteins and adapter proteins which mediate cell proliferation. Phosphorylation on Tyr-454 is required for PTPN6 interaction, Tyr-426 for PTPN11. Tyr-426 is also required for SOCS3 binding, but Tyr-454/Tyr-456 motif is the preferred binding site. Ubiquitinated by the ECS(SOCS2) complex following ligand-binding and phosphorylation by JAK2, leading to its degradation by the proteasome. Regulation by the ECS(SOCS2) complex acts as a negative feedback loop of erythropoietin-mediated signaling pathway. Ubiquitination at Lys-281 mediates receptor internalization, whereas ubiquitination at Lys-453 promotes trafficking of activated receptors to the lysosomes for degradation. Ubiquitinated by NOSIP.appears to be either multi-monoubiquitinated or polyubiquitinated. Ubiquitination mediates proliferation and survival of EPO-dependent cells. |
| Function | Receptor for erythropoietin, which mediates erythropoietin-induced erythroblast proliferation and differentiation. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. May also activate the LYN tyrosine kinase. Isoform EPOR-T: Acts as a dominant-negative receptor of EPOR-mediated signaling. |
| Protein Name | Erythropoietin ReceptorEpo-R |
| Database Links | Reactome: R-HSA-9006335Reactome: R-HSA-9027276Reactome: R-HSA-9027277Reactome: R-HSA-9027283Reactome: R-HSA-9027284 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinIsoform Epor-S: SecretedSecreted And Located To The Cell Surface |
| Alternative Antibody Names | Anti-Erythropoietin Receptor antibodyAnti-Epo-R antibodyAnti-EPOR antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
