• Immunohistochemistry analysis of EPN1 in paraffin-embedded Mouse kidney using EPN1 Rabbit polyclonal antibody (STJ119738) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of EPN1 in paraffin-embedded Rat kidney using EPN1 Rabbit polyclonal antibody (STJ119738) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using EPN1 Rabbit polyclonal antibody (STJ119738) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-EPN1 antibody (130-180) (STJ119738)

SKU:
STJ119738

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EPN1 (130-180) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EPN1
Gene ID: 29924
Uniprot ID: EPN1_HUMAN
Immunogen Region: 130-180
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 130-180 of human EPN1 (NP_037465.2).
Immunogen Sequence: LVALLRDEDRLREERAHALK TKEKLAQTATASSAAVGSGP PPEAEQAWPQS
Post Translational Modifications Phosphorylated on serine and/or threonine residues in mitotic cells. Phosphorylation reduces interaction with REPS2, AP-2 and the membrane fraction. Depolarization of synaptosomes results in dephosphorylation. Ubiquitinated.
Function Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Modifies membrane curvature and facilitates the formation of clathrin-coated invaginations. Regulates receptor-mediated endocytosis.
Protein Name Epsin-1
Eh Domain-Binding Mitotic Phosphoprotein
Eps-15-Interacting Protein 1
Database Links Reactome: R-HSA-182971
Reactome: R-HSA-8856825
Reactome: R-HSA-8856828
Cellular Localisation Cytoplasm
Cell Membrane
Peripheral Membrane Protein
Nucleus
Membrane
Clathrin-Coated Pit
Associated With The Cytoplasmic Membrane At Sites Where Clathrin-Coated Pits Are Forming
Colocalizes With Clathrin And Ap-2 In A Punctate Pattern On The Plasma Membrane
Detected In Presynaptic Nerve Terminals And In Golgi Stacks
May Shuttle To The Nucleus When Associated With Zbtb16/Znf145
Alternative Antibody Names Anti-Epsin-1 antibody
Anti-Eh Domain-Binding Mitotic Phosphoprotein antibody
Anti-Eps-15-Interacting Protein 1 antibody
Anti-EPN1 antibody

Information sourced from Uniprot.org

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