STJ73454 (0. 5µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-EPHX2 antibody (Internal) (STJ73454)

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STJ73454-100
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Host: Goat
Applications: Pep-ELISA/WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description : Goat polyclonal antibody anti-EPHX2 (Internal) is suitable for use in ELISA and Western Blot research applications.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: Peptide ELISA: antibody detection limit dilution 1:2000.
WB: Approx 60kDa band observed in Human Kidney lysates (calculated MW of 57.1kDa according to NP_001243411.1). Recommended concentration: 0.3-1µg/ml.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: EPHX2
Gene ID: 2053
Uniprot ID: HYES_HUMAN
Immunogen Region: Internal
Accession Number: NP_001970.2; NP_001243411.1; NP_001243412.1
Specificity: This antibody is expected to recognize all reported isoforms (NP_001970.2; NP_001243411.1; NP_001243412.1). Reported variants represent identical protein: NP_001243413.1, NP_001243411.1.
Immunogen Sequence: SRTFKSLFRASDES
Function Bifunctional enzyme. The C-terminal domain has epoxide hydrolase activity and acts on epoxides (alkene oxides, oxiranes) and arene oxides. Plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. Also determines steady-state levels of physiological mediators. Bifunctional enzyme. The N-terminal domain has lipid phosphatase activity, with the highest activity towards threo-9,10-phosphonooxy-hydroxy-octadecanoic acid, followed by erythro-9,10-phosphonooxy-hydroxy-octadecanoic acid, 12-phosphonooxy-octadec-9Z-enoic acid and 12-phosphonooxy-octadec-9E-enoic acid. Has phosphatase activity toward lyso-glycerophospholipids with also some lower activity toward lysolipids of sphingolipid and isoprenoid phosphates.
Protein Name Bifunctional Epoxide Hydrolase 2 Includes - Cytosolic Epoxide Hydrolase 2
Ceh
Epoxide Hydratase
Soluble Epoxide Hydrolase
Seh - Lipid-Phosphate Phosphatase
Database Links Reactome: R-HSA-2142670
Reactome: R-HSA-9018682
Reactome: R-HSA-9033241
Cellular Localisation Cytoplasm
Peroxisome
Alternative Antibody Names Anti-Bifunctional Epoxide Hydrolase 2 Includes - Cytosolic Epoxide Hydrolase 2 antibody
Anti-Ceh antibody
Anti-Epoxide Hydratase antibody
Anti-Soluble Epoxide Hydrolase antibody
Anti-Seh - Lipid-Phosphate Phosphatase antibody
Anti-EPHX2 antibody

Information sourced from Uniprot.org

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