• Western blot analysis of extracts of various cell lines, using EPHX2 antibody (STJ23552) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.
  • Immunofluorescence analysis of MCF7 cells using EPHX2 rabbit polyclonal antibody (STJ23552) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using EPHX2 rabbit polyclonal antibody (STJ23552) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using EPHX2 rabbit polyclonal antibody (STJ23552) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-EPHX2 antibody (30-240) (STJ23552)

SKU:
STJ23552

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EPHX2 (30-240) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EPHX2
Gene ID: 2053
Uniprot ID: HYES_HUMAN
Immunogen Region: 30-240
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 30-240 of human EPHX2 (NP_001970.2).
Immunogen Sequence: LALPRGLLNDAFQKGGPEGA TTRLMKGEITLSQWIPLMEE NCRKCSETAKVCLPKNFSIK EIFDKAISARKINRPMLQAA LMLRKKGFTTAILTNTWLDD RAERDGLAQLMCELKMHFDF LIESCQVGMVKPEPQIYKFL LDTLKASPSEVVFLDDIGAN LKPARDLGMVTILVQDTDTA LKELEKVTGIQLLNTPAPLP TSCNPSDMSHG
Post Translational Modifications The N-terminus is blocked. The covalent modification of cysteine by 15-deoxy-Delta12,14-prostaglandin-J2 is autocatalytic and reversible. It may occur as an alternative to other cysteine modifications, such as S-nitrosylation and S-palmitoylation (Probable).
Function Bifunctional enzyme. The C-terminal domain has epoxide hydrolase activity and acts on epoxides (alkene oxides, oxiranes) and arene oxides. Plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. Also determines steady-state levels of physiological mediators. Bifunctional enzyme. The N-terminal domain has lipid phosphatase activity, with the highest activity towards threo-9,10-phosphonooxy-hydroxy-octadecanoic acid, followed by erythro-9,10-phosphonooxy-hydroxy-octadecanoic acid, 12-phosphonooxy-octadec-9Z-enoic acid and 12-phosphonooxy-octadec-9E-enoic acid. Has phosphatase activity toward lyso-glycerophospholipids with also some lower activity toward lysolipids of sphingolipid and isoprenoid phosphates.
Protein Name Bifunctional Epoxide Hydrolase 2 Includes - Cytosolic Epoxide Hydrolase 2
Ceh
Epoxide Hydratase
Soluble Epoxide Hydrolase
Seh - Lipid-Phosphate Phosphatase
Database Links Reactome: R-HSA-2142670
Reactome: R-HSA-9018682
Reactome: R-HSA-9033241
Cellular Localisation Cytoplasm
Peroxisome
Alternative Antibody Names Anti-Bifunctional Epoxide Hydrolase 2 Includes - Cytosolic Epoxide Hydrolase 2 antibody
Anti-Ceh antibody
Anti-Epoxide Hydratase antibody
Anti-Soluble Epoxide Hydrolase antibody
Anti-Seh - Lipid-Phosphate Phosphatase antibody
Anti-EPHX2 antibody

Information sourced from Uniprot.org

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