| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Rat/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Ephrin type-B receptor 2 (991-1040 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000 IHC 1:100-1:300 ELISA 1:5000 IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | EPHB2 |
| Gene ID: | 2048 |
| Uniprot ID: | EPHB2_HUMAN |
| Immunogen Region: | 991-1040 aa |
| Specificity: | EphB2 Polyclonal Antibody detects endogenous levels of EphB2 protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human EPHB2 at the amino acid range 991-1040 |
| Post Translational Modifications | Autophosphorylated.ligand binding stimulates autophosphorylation on tyrosine residues. Polyubiquitinated.ligand binding stimulates ubiquitination. Ubiquitinated by RNF186 at Lys-891, mainly through 'Lys-27'-linked polyubiquitin chains. Ligand binding induces cleavage by matrix metalloproteinases (MMPs) such as MMP7/MMP9, producing an EphB2/N-terminal fragment (NTF) and a C-terminal long fragment (EphB2-LF). EphB2-LF is further cleaved by MMPs, producing EphB2/CTF1 which is further cleaved by the PS1/gamma-secretase producing EphB2/CTF2. |
| Function | Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Functions in axon guidance during development. Involved in the guidance of commissural axons, that form a major interhemispheric connection between the 2 temporal lobes of the cerebral cortex. Also involved in guidance of contralateral inner ear efferent growth cones at the midline and of retinal ganglion cell axons to the optic disk. In addition to axon guidance, also regulates dendritic spines development and maturation and stimulates the formation of excitatory synapses. Upon activation by EFNB1, abolishes the ARHGEF15-mediated negative regulation on excitatory synapse formation. Controls other aspects of development including angiogenesis, palate development and in inner ear development through regulation of endolymph production. Forward and reverse signaling through the EFNB2/EPHB2 complex regulate movement and adhesion of cells that tubularize the urethra and septate the cloaca. May function as a tumor suppressor. May be involved in the regulation of platelet activation and blood coagulation. |
| Protein Name | Ephrin Type-B Receptor 2 Developmentally-Regulated Eph-Related Tyrosine Kinase Elk-Related Tyrosine Kinase Eph Tyrosine Kinase 3 Eph-Like Kinase 5 Ek5 Hek5 Renal Carcinoma Antigen Ny-Ren-47 Tyrosine-Protein Kinase Tyro5 Tyrosine-Protein Kinase Receptor Eph-3 Cleaved Into - Ephb2/Ctf1 - Ephb2/Ctf2 |
| Database Links | Reactome: R-HSA-2682334 Reactome: R-HSA-373760 Reactome: R-HSA-3928662 Reactome: R-HSA-3928664 Reactome: R-HSA-3928665 |
| Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein Cell Projection Axon Dendrite |
| Alternative Antibody Names | Anti-Ephrin Type-B Receptor 2 antibody Anti-Developmentally-Regulated Eph-Related Tyrosine Kinase antibody Anti-Elk-Related Tyrosine Kinase antibody Anti-Eph Tyrosine Kinase 3 antibody Anti-Eph-Like Kinase 5 antibody Anti-Ek5 antibody Anti-Hek5 antibody Anti-Renal Carcinoma Antigen Ny-Ren-47 antibody Anti-Tyrosine-Protein Kinase Tyro5 antibody Anti-Tyrosine-Protein Kinase Receptor Eph-3 Cleaved Into - Ephb2/Ctf1 - Ephb2/Ctf2 antibody Anti-EPHB2 antibody Anti-DRT antibody Anti-EPHT3 antibody Anti-EPTH3 antibody Anti-ERK antibody Anti-HEK5 antibody Anti-TYRO5 antibody |
Information sourced from Uniprot.org