| Host: | Goat |
| Applications: | Pep-ELISA/WB |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Goat polyclonal antibody anti-EPCAM/TROP1 (167-179) is suitable for use in ELISA and Western Blot research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:128000. WB: Approx 37kDa band observed in lysates of cell line CACO-2 (calculated MW of 34.9kDa according to NP_002345.2). Recommended concentration: 0.03-0.1µg/ml. |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | EPCAM |
| Gene ID: | 4072 |
| Uniprot ID: | EPCAM_HUMAN |
| Immunogen Region: | 167-179 |
| Accession Number: | NP_002345.2 |
| Immunogen Sequence: | QKEITTRYQLDPK |
| Post Translational Modifications | Hyperglycosylated in carcinoma tissue as compared with autologous normal epithelia. Glycosylation at Asn-198 is crucial for protein stability. |
| Function | May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E. |
| Protein Name | Epithelial Cell Adhesion Molecule Ep-Cam Adenocarcinoma-Associated Antigen Cell Surface Glycoprotein Trop-1 Epithelial Cell Surface Antigen Epithelial Glycoprotein Egp Epithelial Glycoprotein 314 Egp314 Hegp314 Ks 1/4 Antigen Ksa Major Gastrointestinal Tumor-Associated Protein Ga733-2 Tumor-Associated Calcium Signal Transducer 1 Cd Antigen Cd326 |
| Database Links | Reactome: R-HSA-202733 |
| Cellular Localisation | Lateral Cell Membrane Single-Pass Type I Membrane Protein Cell Junction Tight Junction Colocalizes With Cldn7 At The Lateral Cell Membrane And Tight Junction |
| Alternative Antibody Names | Anti-Epithelial Cell Adhesion Molecule antibody Anti-Ep-Cam antibody Anti-Adenocarcinoma-Associated Antigen antibody Anti-Cell Surface Glycoprotein Trop-1 antibody Anti-Epithelial Cell Surface Antigen antibody Anti-Epithelial Glycoprotein antibody Anti-Egp antibody Anti-Epithelial Glycoprotein 314 antibody Anti-Egp314 antibody Anti-Hegp314 antibody Anti-Ks 1/4 Antigen antibody Anti-Ksa antibody Anti-Major Gastrointestinal Tumor-Associated Protein Ga733-2 antibody Anti-Tumor-Associated Calcium Signal Transducer 1 antibody Anti-Cd Antigen Cd326 antibody Anti-EPCAM antibody Anti-GA733-2 antibody Anti-M1S2 antibody Anti-M4S1 antibody Anti-MIC18 antibody Anti-TACSTD1 antibody Anti-TROP1 antibody |
Information sourced from Uniprot.org
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