• Western blot analysis of various lysates, using EPB42 antibody (STJ119585) at 1:800 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45s.

Anti-EPB42 antibody (10-240) (STJ119585)

SKU:
STJ119585

£112.50 - £524.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EPB42
Gene ID: 2038
Uniprot ID: EPB42_HUMAN
Immunogen Region: 10-240
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 10-240 of human EPB42 (NP_001107606).
Immunogen Sequence: CDFQAARNNEEHHTKALSSR RLFVRRGQPFTIILYFRAPV RAFLPALKKVALTAQTGEQP SKINRTQATFPISSLGDRKW WSAVVEERDAQSWTISVTTP ADAVIGHYSLLLQVSGRKQL LLGQFTLLFNPWNREDAVFL KNEAQRMEYLLNQNGLIYLG TADCIQAESWDFGQFEGDVI DLSLRLLSKDKQVEKWSQPV HVARVLGALLHFLKEQRVLP TPQTQATQEGA
Post Translational Modifications Both cAMP-dependent kinase (CAPK) and another kinase present in the red-blood cells seem to be able to phosphorylate EPB42.
Function Component of the ankyrin-1 complex, a multiprotein complex involved in the stability and shape of the erythrocyte membrane.
Protein Name Protein 4.2
P4.2
Erythrocyte Membrane Protein Band 4.2
Erythrocyte Protein 4.2
Cellular Localisation Cell Membrane
Lipid-Anchor
Cytoplasmic Side
Cytoplasm
Cytoskeleton
Cytoplasmic Surface Of Erythrocyte Membranes
Alternative Antibody Names Anti-Protein 4.2 antibody
Anti-P4.2 antibody
Anti-Erythrocyte Membrane Protein Band 4.2 antibody
Anti-Erythrocyte Protein 4.2 antibody
Anti-EPB42 antibody
Anti-E42P antibody

Information sourced from Uniprot.org

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