• STJ71353 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ71353 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cell junction staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ71353 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-EPB41L2/4.1G antibody (593-604) (STJ71353)

SKU:
STJ71353-100

£303.50
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Host: Goat
Applications: Pep-ELISA/IF/IHC/FC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-EPB41L2/4.1G (593-604) is suitable for use in ELISA, Immunofluorescence, Immunohistochemistry and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IHC-2.5µg/ml
IF-Strong expression of the protein seen in the membranes of A431 and in the cell junctions of U2OS cells. 10µg/ml
ELISA-antibody detection limit dilution 1:32000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: EPB41L2
Gene ID: 2037
Uniprot ID: E41L2_HUMAN
Immunogen Region: 593-604
Accession Number: NP_001422.1; NP_001129026.1; NP_001186317.1; NP_001239589.1; NP_001337228.1; NP_001337230.1; NP_001337232.1; NP_001337233.1; NP_001337236.1; NP_001337243.1; NP_001337249.1
Specificity: This antibody is reported to recognise all isoforms NP_001422.1 (iso a) , NP_001129026.1 (iso b) , NP_001186317.1 (iso c) , NP_001239589.1 (iso d) , NP_001337228.1 (iso e) , NP_001337230.1 (iso f) , NP_001337232.1 (iso g) , NP_001337233.1 (iso h) , N
Immunogen Sequence: RREVRSPTKAPH
Function Required for dynein-dynactin complex and NUMA1 recruitment at the mitotic cell cortex during anaphase.
Protein Name Band 4.1-Like Protein 2
Erythrocyte Membrane Protein Band 4.1-Like 2
Generally Expressed Protein 4.1
4.1g
Database Links Reactome: R-HSA-6794361
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Cortex
Cell Membrane
Alternative Antibody Names Anti-Band 4.1-Like Protein 2 antibody
Anti-Erythrocyte Membrane Protein Band 4.1-Like 2 antibody
Anti-Generally Expressed Protein 4.1 antibody
Anti-4.1g antibody
Anti-EPB41L2 antibody

Information sourced from Uniprot.org

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