• Western blot analysis of various lysates, using EPB41 Rabbit polyclonal antibody (STJ23548) at 1:700 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-EPB41 antibody (210-500) (STJ23548)

SKU:
STJ23548

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EPB41 (210-500) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EPB41
Gene ID: 2035
Uniprot ID: EPB41_HUMAN
Immunogen Region: 210-500
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 210-500 of human EPB41 (NP_001159478.1).
Immunogen Sequence: MHCKVSLLDDTVYECVVEKH AKGQDLLKRVCEHLNLLEED YFGLAIWDNATSKTWLDSAK EIKKQVRGVPWNFTFNVKFY PPDPAQLTEDITRYYLCLQL RQDIVAGRLPCSFATLALLG SYTIQSELGDYDPELHGVDY VSDFKLAPNQTKELEEKVME LHKSYRSMTPAQADLEFLEN AKKLSMYGVDLHKAKDLEGV DIILGVCSSGLLVYKDKLRI NRFPWPKVLKISYKRSSFF
Post Translational Modifications Phosphorylated at multiple sites by different protein kinases and each phosphorylation event selectively modulates the protein's functions. Phosphorylation on Tyr-660 reduces the ability of 4.1 to promote the assembly of the spectrin/actin/4.1 ternary complex. O-glycosylated.contains N-acetylglucosamine side chains in the C-terminal domain.
Function Protein 4.1 is a major structural element of the erythrocyte membrane skeleton. It plays a key role in regulating membrane physical properties of mechanical stability and deformability by stabilizing spectrin-actin interaction. Recruits DLG1 to membranes. Required for dynein-dynactin complex and NUMA1 recruitment at the mitotic cell cortex during anaphase.
Protein Name Protein 4.1
P4.1
4.1r
Band 4.1
Epb4.1
Erythrocyte Membrane Protein Band 4.1
Database Links Reactome: R-HSA-6794361
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Cortex
Nucleus
Alternative Antibody Names Anti-Protein 4.1 antibody
Anti-P4.1 antibody
Anti-4.1r antibody
Anti-Band 4.1 antibody
Anti-Epb4.1 antibody
Anti-Erythrocyte Membrane Protein Band 4.1 antibody
Anti-EPB41 antibody
Anti-E41P antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance