| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 IHC-P:1:50-1:200 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | EPAS1 |
| Gene ID: | 2034 |
| Uniprot ID: | EPAS1_HUMAN |
| Immunogen Region: | 678-745 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 678-745 of human EPAS1/HIF2 Alpha (NP_001421.2). |
| Immunogen Sequence: | TFKTRSAKGFGARGPDVLSP AMVALSNKLKLKRQLEYEEQ AFQDLSGGDPPGGSTSHLMW KRMKNLRG |
| Tissue Specificity | Expressed in most tissues, with highest levels in placenta, lung and heart. Selectively expressed in endothelial cells. |
| Post Translational Modifications | In normoxia, is probably hydroxylated on Pro-405 and Pro-531 by EGLN1/PHD1, EGLN2/PHD2 and/or EGLN3/PHD3. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization. In normoxia, is hydroxylated on Asn-847 by HIF1AN thus probably abrogating interaction with CREBBP and EP300 and preventing transcriptional activation. Phosphorylated on multiple sites in the CTAD. The iron and 2-oxoglutarate dependent 3-hydroxylation of asparagine is (S) stereospecific within HIF CTAD domains. |
| Function | Transcription factor involved in the induction of oxygen regulated genes. Heterodimerizes with ARNT.heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation requires recruitment of transcriptional coactivators such as CREBBP and probably EP300. Interaction with redox regulatory protein APEX1 seems to activate CTAD. |
| Protein Name | Endothelial Pas Domain-Containing Protein 1 Epas-1 Basic-Helix-Loop-Helix-Pas Protein Mop2 Class E Basic Helix-Loop-Helix Protein 73 Bhlhe73 Hif-1-Alpha-Like Factor Hlf Hypoxia-Inducible Factor 2-Alpha Hif-2-Alpha Hif2-Alpha Member Of Pas Protein 2 Pas Domain-Containing Protein 2 |
| Database Links | Reactome: R-HSA-1234158 Reactome: R-HSA-1234174 Reactome: R-HSA-1234176 Reactome: R-HSA-452723 Reactome: R-HSA-8849473 Reactome: R-HSA-8951664 Reactome: R-HSA-9664873 |
| Cellular Localisation | Nucleus Nucleus Speckle Colocalizes With Hif3a In The Nucleus And Speckles |
| Alternative Antibody Names | Anti-Endothelial Pas Domain-Containing Protein 1 antibody Anti-Epas-1 antibody Anti-Basic-Helix-Loop-Helix-Pas Protein Mop2 antibody Anti-Class E Basic Helix-Loop-Helix Protein 73 antibody Anti-Bhlhe73 antibody Anti-Hif-1-Alpha-Like Factor antibody Anti-Hlf antibody Anti-Hypoxia-Inducible Factor 2-Alpha antibody Anti-Hif-2-Alpha antibody Anti-Hif2-Alpha antibody Anti-Member Of Pas Protein 2 antibody Anti-Pas Domain-Containing Protein 2 antibody Anti-EPAS1 antibody Anti-BHLHE73 antibody Anti-HIF2A antibody Anti-MOP2 antibody Anti-PASD2 antibody |
Information sourced from Uniprot.org