Anti-ENPP2/AUTOTAXIN antibody (698-712) (STJ73041)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-ENPP2/AUTOTAXIN (698-712) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Applications | Pep-ELISA/WB/IHC |
| Host | Goat |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 100kDa band observed in Human Placenta lysates (calculated MW of 105kDa according to NP_006200.3). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour.IHC |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | ENPP2 |
| Gene ID | 5168 |
| Uniprot ID | ENPP2_HUMAN |
| Accession Number | NP_006200.3; NP_001035181.1, NP_001124335.1 |
| Immunogen Region | 698-712 |
| Immunogen Sequence | PDHLTSCVRPDVRVS |
| Specificity | This antibody is expected to recognize iall reported isoforms (NP_006200.3; NP_001035181.1, NP_001124335.1). |
Additional Info
| Post Translational Modifications | N-glycosylation, but not furin-cleavage, plays a critical role on secretion and on lysoPLD activity. The interdomain disulfide bond between Cys-414 and Cys-806 is essential for catalytic activity. |
| Function | Secreted lysophospholipase D that hydrolyzes lysophospholipids to produce the signaling molecule lysophosphatidic acid (LPA) in extracellular fluids. Its major substrate is lysophosphatidylcholine. Can also act on sphingosylphosphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP. Involved in several motility-related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation. Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein. May have a role in induction of parturition. Possible involvement in cell proliferation and adipose tissue development (Probable). Required for LPA production in activated platelets, cleaves the sn-1 lysophospholipids to generate sn-1 lysophosphatidic acids containing predominantly 18:2 and 20:4 fatty acids. Shows a preference for the sn-1 to the sn-2 isomer of 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF). |
| Protein Name | AutotaxinEctonucleotide Pyrophosphatase/Phosphodiesterase Family Member 2E-Npp 2Extracellular Lysophospholipase DLysopld |
| Database Links | Reactome: R-HSA-199220 |
| Cellular Localisation | Secreted |
| Alternative Antibody Names | Anti-Autotaxin antibodyAnti-Ectonucleotide Pyrophosphatase/Phosphodiesterase Family Member 2 antibodyAnti-E-Npp 2 antibodyAnti-Extracellular Lysophospholipase D antibodyAnti-Lysopld antibodyAnti-ENPP2 antibodyAnti-ATX antibodyAnti-PDNP2 antibody |
Information sourced from Uniprot.org