| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-ENPP2 (564-863) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ENPP2 |
| Gene ID: |
5168 |
| Uniprot ID: |
ENPP2_HUMAN |
| Immunogen Region: |
564-863 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 564-863 of human ENPP2 (NP_001035181.1). |
| Immunogen Sequence: |
DLGCTCDDKVEPKNKLDELN KRLHTKGSTEERHLLYGRPA VLYRTRYDILYHTDFESGYS EIFLMPLWTSYTVSKQAEVS SVPDHLTSCVRPDVRVSPSF SQNCLAYKNDKQMSYGFLFP PYLSSSPEAKYDAFLVTNMV PMYPAFKRVWNYFQRVLVKK YASERNGVNVISGPIFDYDY DGLHDTEDKIKQYVEGSSIP VPTHYYSIITSCLDFTQPAD KCDGPLSVSSFILPHRPDN |
| Tissue Specificity | Detected in blood plasma (at protein level). Predominantly expressed in brain, placenta, ovary, and small intestine. Expressed in a number of carcinomas such as hepatocellular and prostate carcinoma, neuroblastoma and non-small-cell lung cancer. Expressed in body fluids such as plasma, cerebral spinal fluid (CSF), saliva, follicular and amniotic fluids. Not detected in leukocytes. Isoform 1 is more highly expressed in peripheral tissues than in the central nervous system (CNS). Adipocytes only express isoform 1. Isoform 3 is more highly expressed in the brain than in peripheral tissues. |
| Post Translational Modifications | N-glycosylation, but not furin-cleavage, plays a critical role on secretion and on lysoPLD activity. The interdomain disulfide bond between Cys-414 and Cys-806 is essential for catalytic activity. |
| Function | Hydrolyzes lysophospholipids to produce the signaling molecule lysophosphatidic acid (LPA) in extracellular fluids. Major substrate is lysophosphatidylcholine. Can also act on sphingosylphosphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP. Involved in several motility-related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation. Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein. May have a role in induction of parturition. Possible involvement in cell proliferation and adipose tissue development (Probable). Tumor cell motility-stimulating factor. Required for LPA production in activated platelets, cleaves the sn-1 lysophospholipids to generate sn-1 lysophosphatidic acids containing predominantly 18:2 and 20:4 fatty acids. Shows a preference for the sn-1 to the sn-2 isomer of 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF). |
| Protein Name | Ectonucleotide Pyrophosphatase/Phosphodiesterase Family Member 2E-Npp 2AutotaxinExtracellular Lysophospholipase DLysopld |
| Database Links | Reactome: R-HSA-199220 |
| Cellular Localisation | Secreted |
| Alternative Antibody Names | Anti-Ectonucleotide Pyrophosphatase/Phosphodiesterase Family Member 2 antibodyAnti-E-Npp 2 antibodyAnti-Autotaxin antibodyAnti-Extracellular Lysophospholipase D antibodyAnti-Lysopld antibodyAnti-ENPP2 antibodyAnti-ATX antibodyAnti-PDNP2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
