Anti-eNOS antibody (C-Term) (STJA0041297)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThis antibody was generated from a recombinant mouse eNOS protein that included amino acids residues in the C-terminal region. This sequence is conserved in human and rat eNOS, and has low homology to other NOS family member
General Information
| Short Description | Rabbit polyclonal anti-eNOS (C-Term) for use in ELISA, WB, ICC and IHC in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | ELISA/WB/ICC/IHC |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Antigen Affinity Purified |
| Dilution Range | WB 1:1000IHC 1:100ICC 1:50 |
| Formulation | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Instruction | Storage at-20ยฐC is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at-20ยฐC. |
Target Information
| Gene Symbol | Nos3 |
| Gene ID | 18127 |
| Uniprot ID | NOS3_MOUSE |
| Immunogen | This antibody was generated from a recombinant mouse eNOS protein that included amino acids residues in the C-terminal region. This sequence is conserved in human and rat eNOS, and has low homology to other NOS family member |
| Immunogen Region | C-Term |
| Immunogen Sequence | Mouse |
| Specificity | The antibody detects a 140 kDa* protein corresponding to eNOS on SDS-PAGE immunoblots of human umbilical vein endothelial cells. |
Additional Info
| Post Translational Modifications | Phosphorylation by AMPK at Ser-1176 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-494, resulting in inhibition of activity. |
| Function | Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. May play a significant role in normal and abnormal limb development. |
| Protein Name | Nitric Oxide Synthase 3Constitutive NosCnosEc-NosNos Type IiiNosiiiNitric Oxide Synthase - EndothelialEndothelial NosEnos |
| Database Links | Reactome: R-MMU-1222556Reactome: -MMU-1474151Reactome: -MMU-203615Reactome: -MMU-203641Reactome: -MMU-203754Reactome: -MMU-392154Reactome: -MMU-5218920Reactome: -MMU-9009391Reactome: -MMU-9856530 |
| Cellular Localisation | MembraneCaveolaCytoplasmCytoskeletonGolgi ApparatusCell MembraneSpecifically Associates With Actin Cytoskeleton In The G2 Phase Of The Cell CycleWhich Is Favored By Interaction With Nosip And Results In A Reduced Enzymatic Activity |
| Alternative Antibody Names | Anti-Nitric Oxide Synthase 3 antibodyAnti-Constitutive Nos antibodyAnti-Cnos antibodyAnti-Ec-Nos antibodyAnti-Nos Type Iii antibodyAnti-Nosiii antibodyAnti-Nitric Oxide Synthase - Endothelial antibodyAnti-Endothelial Nos antibodyAnti-Enos antibodyAnti-Nos3 antibodyAnti-Ecnos antibody |
Information sourced from Uniprot.org