Western blot analysis of extracts of various cell lines, using ENO2 antibody (STJ11101628) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-ENO2 antibody (47-100) [S8MR] (STJ11101628)

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STJ11101628
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Monoclonal
Clone ID : S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:1000-1:6000
IHC-P:1:500-1:2000
IF/ICC:1:100-1:800
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ENO2
Gene ID: 2026
Uniprot ID: ENOG_HUMAN
Immunogen Region: 47-100
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 47-100 of human ENO2 (NP_001966.1).
Immunogen Sequence: LELRDGDKQRYLGKGVLKAV DHINSTIAPALISSGLSVVE QEKLDNLMLELDGTENKSKF GANA
Tissue Specificity The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
Function Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
Protein Name Gamma-Enolase
2-Phospho-D-Glycerate Hydro-Lyase
Enolase 2
Neural Enolase
Neuron-Specific Enolase
Nse
Database Links Reactome: R-HSA-70171
Reactome: R-HSA-70263
Cellular Localisation Cytoplasm
Cell Membrane
Can Translocate To The Plasma Membrane In Either The Homodimeric (Alpha/Alpha) Or Heterodimeric (Alpha/Gamma) Form
Alternative Antibody Names Anti-Gamma-Enolase antibody
Anti-2-Phospho-D-Glycerate Hydro-Lyase antibody
Anti-Enolase 2 antibody
Anti-Neural Enolase antibody
Anti-Neuron-Specific Enolase antibody
Anti-Nse antibody
Anti-ENO2 antibody

Information sourced from Uniprot.org

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