• Western blot analysis of extracts of SH-SY5Y cells, using Engrailed 1 Rabbit monoclonal antibody (STJ11103080) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of extracts of various cell lines, using Engrailed 1 Rabbit monoclonal antibody (STJ11103080) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Engrailed 1 antibody (293-392) [S0MR] (STJ11103080)

SKU:
STJ11103080

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Engrailed 1 (293-392) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EN1
Gene ID: 2019
Uniprot ID: HME1_HUMAN
Immunogen Region: 293-392
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 293-392 of human Engrailed 1 (Q05925).
Immunogen Sequence: KLKKKKNEKEDKRPRTAFTA EQLQRLKAEFQANRYITEQR RQTLAQELSLNESQIKIWFQ NKRAKIKKATGIKNGLALHL MAQGLYNHSTTTVQDKDESE
Function Required for proper formation of the apical ectodermal ridge and correct dorsal-ventral patterning in the limb.
Protein Name Homeobox Protein Engrailed-1
Homeobox Protein En-1
Hu-En-1
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Homeobox Protein Engrailed-1 antibody
Anti-Homeobox Protein En-1 antibody
Anti-Hu-En-1 antibody
Anti-EN1 antibody

Information sourced from Uniprot.org

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