| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Protein enabled homolog (472-521 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:10000IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | ENAH |
| Gene ID: | 55740 |
| Uniprot ID: | ENAH_HUMAN |
| Immunogen Region: | 472-521 aa |
| Specificity: | Mena Polyclonal Antibody detects endogenous levels of Mena protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human ENAH at the amino acid range 472-521 |
| Post Translational Modifications | NTN1-induced PKA phosphorylation on Ser-265 directly parallels the formation of filopodial protrusions. |
| Function | Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. ENAH induces the formation of F-actin rich outgrowths in fibroblasts. Acts synergistically with BAIAP2-alpha and downstream of NTN1 to promote filipodia formation. |
| Protein Name | Protein Enabled Homolog |
| Database Links | Reactome: R-HSA-202433Reactome: R-HSA-376176 |
| Cellular Localisation | CytoplasmCytoskeletonCell ProjectionLamellipodiumFilopodiumSynapseCell JunctionFocal AdhesionTargeted To The Leading Edge Of Lamellipodia And Filopodia By Mrl Family MembersColocalizes At Filopodial Tips With A Number Of Other Proteins Including Vinculin And ZyxlinColocalizes With N-Wasp At The Leading EdgeColocalizes With Gphn And Pfn At Synapses |
| Alternative Antibody Names | Anti-Protein Enabled Homolog antibodyAnti-ENAH antibodyAnti-MENA antibody |
Information sourced from Uniprot.org