| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | ELP5 |
| Gene ID: | 23587 |
| Uniprot ID: | ELP5_HUMAN |
| Immunogen Region: | 17-316 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 17-316 of human ELP5 (NP_056177.3). |
| Immunogen Sequence: | MLDSLLALGGLVLLRDSVEW EGRSLLKALVKKSALCGEQV HILGCEVSEEEFREGFDSDI NNRLVYHDFFRDPLNWSKTE EAFPGGPLGALRAMCKRTDP VPVTIALDSLSWLLLRLPCT TLCQVLHAVSHQDSCPGDSS SVGKVSVLGLLHEELHGPGP VGALSSLAQTEVTLGGTMGQ ASAHILCRRPRQRPTDQTQW FSILPDFSLDLQEGPSVESQ PYSDPHIPPVDPTTHLTFN |
| Tissue Specificity | Ubiquitously expressed with high levels in heart, brain, liver, skeletal muscle and testis. |
| Post Translational Modifications | Tyrosine-phosphorylated. |
| Function | Component of the elongator complex which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine). The elongator complex catalyzes formation of carboxymethyluridine in the wobble base at position 34 in tRNAs. Involved in cell migration. |
| Protein Name | Elongator Complex Protein 5Dermal Papilla-Derived Protein 6S-Phase 2 Protein |
| Database Links | Reactome: R-HSA-3214847 |
| Cellular Localisation | NucleusCytoplasm |
| Alternative Antibody Names | Anti-Elongator Complex Protein 5 antibodyAnti-Dermal Papilla-Derived Protein 6 antibodyAnti-S-Phase 2 Protein antibodyAnti-ELP5 antibodyAnti-C17orf81 antibodyAnti-DERP6 antibodyAnti-HSPC002 antibodyAnti-MSTP071 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and AKR1B1 knockout (KO) HeLa cells, using [KO Validated] AKR1B1 Rabbit polyclonal antibody (STJ11100004) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89077/357534/STJ11100004_1__87524.1713121649.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and AKR1B1 knockout (KO) HeLa cells, using [KO Validated] AKR1B1 Rabbit polyclonal antibody (STJ11100004) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
