| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Rabbit polyclonal antibody anti-Elongin-C (40-120 aa) is suitable for use in Western Blot and ELISA research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-2000 ELISA 1:5000-20000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | ELOC |
| Gene ID: | 6921 |
| Uniprot ID: | ELOC_HUMAN |
| Immunogen Region: | 40-120 aa |
| Specificity: | ELOC Polyclonal Antibody detects endogenous levels of protein. |
| Immunogen: | Synthesized peptide derived from the human protein at the amino acid range 40-120 |
| Post Translational Modifications | Ubiquitinated by the DCX(AMBRA1) complex, leading to its degradation by the proteasome. |
| Function | SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex). In embryonic stem cells, the elongin BC complex is recruited by EPOP to Polycomb group (PcG) target genes in order generate genomic region that display both active and repressive chromatin properties, an important feature of pluripotent stem cells. Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. By binding to BC-box motifs it seems to link target recruitment subunits, like VHL and members of the SOCS box family, to Cullin/RBX1 modules that activate E2 ubiquitination enzymes. Component the von Hippel-Lindau ubiquitination complex CBC(VHL). A number of ECS complexes (containing either KLHDC2, KLHDC3, KLHDC10, APPBP2, FEM1A, FEM1B or FEM1C as substrate-recognition component) are part of the DesCEND (destruction via C-end degrons) pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation. The ECS(ASB9) complex mediates ubiquitination and degradation of CKB. As part of a multisubunit ubiquitin ligase complex, polyubiquitinates monoubiquitinated POLR2A. ECS(LRR1) ubiquitinates MCM7 and promotes CMG replisome disassembly by VCP and chromatin extraction during S-phase. (Microbial infection) Following infection by HIV-1 virus, component of a cullin-5-RING E3 ubiquitin-protein ligase complex (ECS complex) hijacked by the HIV-1 Vif protein, which catalyzes ubiquitination and degradation of APOBEC3F and APOBEC3G. The complex can also ubiquitinate APOBEC3H to some extent. |
| Protein Name | Elongin-C Eloc Elongin 15 Kda Subunit Rna Polymerase Ii Transcription Factor Siii Subunit C Siii P15 Transcription Elongation Factor B Polypeptide 1 |
| Database Links | Reactome: R-HSA-112382 Reactome: R-HSA-1234176 Reactome: R-HSA-167152 Reactome: R-HSA-167200 Reactome: R-HSA-167238 Reactome: R-HSA-167243 Reactome: R-HSA-167246 Reactome: R-HSA-167287 Reactome: R-HSA-167290 Reactome: R-HSA-180585 Reactome: R-HSA-674695 Reactome: R-HSA-6796648 Reactome: R-HSA-75955 Reactome: R-HSA-8951664 Reactome: R-HSA-9010553 Reactome: R-HSA-9705462 Reactome: R-HSA-983168 Reactome: R-HSA-9833109 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Elongin-C antibody Anti-Eloc antibody Anti-Elongin 15 Kda Subunit antibody Anti-Rna Polymerase Ii Transcription Factor Siii Subunit C antibody Anti-Siii P15 antibody Anti-Transcription Elongation Factor B Polypeptide 1 antibody Anti-ELOC antibody Anti-TCEB1 antibody |
Information sourced from Uniprot.org
