• Western blot analysis of various lysates using ELMO1 Rabbit polyclonal antibody (STJ117030) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-ELMO1 antibody (527-727) (STJ117030)

SKU:
STJ117030

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ELMO1 (527-727) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ELMO1
Gene ID: 9844
Uniprot ID: ELMO1_HUMAN
Immunogen Region: 527-727
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 527-727 of human ELMO1 (NP_055615.8).
Immunogen Sequence: DFQSRPILELKEKIQPEILE LIKQQRLNRLVEGTCFRKLN ARRRQDKFWYCRLSPNHKVL HYGDLEESPQGEVPHDSLQD KLPVADIKAVVTGKDCPHMK EKGALKQNKEVLELAFSILY DSNCQLNFIAPDKHEYCIWT DGLNALLGKDMMSDLTRNDL DTLLSMEIKLRLLDLENIQI PDAPPPIPKEPSNYDFVYDC N
Tissue Specificity Widely expressed, with a higher expression in the spleen and placenta.
Post Translational Modifications Phosphorylated by HCK.
Function Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in association with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1.
Protein Name Engulfment And Cell Motility Protein 1
Protein Ced-12 Homolog
Database Links Reactome: R-HSA-164944
Reactome: R-HSA-2029482
Reactome: R-HSA-4420097
Reactome: R-HSA-8849471
Reactome: R-HSA-9664422
Cellular Localisation Cytoplasm
Cell Membrane
Translocation To Plasma Membrane Seems To Be Mediated By Dock1 And Crk
Alternative Antibody Names Anti-Engulfment And Cell Motility Protein 1 antibody
Anti-Protein Ced-12 Homolog antibody
Anti-ELMO1 antibody
Anti-KIAA0281 antibody

Information sourced from Uniprot.org

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