Host: |
Rabbit |
Applications: |
WB/IHC/IF |
Reactivity: |
Human/Mouse/Rat |
Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: |
Rabbit polyclonal antibody anti-ELANE (30-267) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
Clonality: |
Polyclonal |
Conjugation: |
Unconjugated |
Isotype: |
IgG |
Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
Purification: |
Affinity purification |
Dilution Range: |
WB 1:500-1:1000IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Gene Symbol: |
ELANE |
Gene ID: |
1991 |
Uniprot ID: |
ELNE_HUMAN |
Immunogen Region: |
30-267 |
Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 30-267 of human Neutrophil Elastase (ELANE) (NP_001963.1). |
Immunogen Sequence: |
IVGGRRARPHAWPFMVSLQL RGGHFCGATLIAPNFVMSAA HCVANVNVRAVRVVLGAHNL SRREPTRQVFAVQRIFENGY DPVNLLNDIVILQLNGSATI NANVQVAQLPAQGRRLGNGV QCLAMGWGLLGRNRGIASVL QELNVTVVTSLCRRSNVCTL VRGRQAGVCFGDSGSPLVCN GLIHGIASFVRGGCASGLYP DAFAPVAQFVNWIDSIIQRS EDNPCPHPRDPDPASRTH |
Tissue Specificity | Bone marrow cells. Neutrophil. |
Function | Serine protease that modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis. Promotes cleavage of GSDMB, thereby inhibiting pyroptosis. Capable of killing E.coli but not S.aureus in vitro.digests outer membrane protein A (ompA) in E.coli and K.pneumoniae. |
Protein Name | Neutrophil ElastaseBone Marrow Serine ProteaseElastase-2Human Leukocyte ElastaseHleMedullasinPmn Elastase |
Database Links | Reactome: R-HSA-1442490Reactome: R-HSA-1474228Reactome: R-HSA-1592389Reactome: R-HSA-5620971Reactome: R-HSA-6798695Reactome: R-HSA-6803157Reactome: R-HSA-977606 |
Cellular Localisation | Cytoplasmic VesiclePhagosomeLocalized In Phagolysosomes Following Ingestion Of EColi By Neutrophils |
Alternative Antibody Names | Anti-Neutrophil Elastase antibodyAnti-Bone Marrow Serine Protease antibodyAnti-Elastase-2 antibodyAnti-Human Leukocyte Elastase antibodyAnti-Hle antibodyAnti-Medullasin antibodyAnti-Pmn Elastase antibodyAnti-ELANE antibodyAnti-ELA2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance