• Western blot analysis of THP-1, using Neutrophil Elastase (ELANE) rabbit polyclonal antibody (STJ114982) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using Neutrophil Elastase (ELANE) rabbit polyclonal antibody (STJ114982) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human esophagus using Neutrophil Elastase (ELANE) rabbit polyclonal antibody (STJ114982) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat bone marrow using Neutrophil Elastase (ELANE) rabbit polyclonal antibody (STJ114982) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of mouse bone marrow cells using Neutrophil Elastase (ELANE) rabbit polyclonal antibody (STJ114982) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat bone marrow cells using Neutrophil Elastase (ELANE) rabbit polyclonal antibody (STJ114982) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of THP-1 cells using Neutrophil Elastase (ELANE) rabbit polyclonal antibody (STJ114982) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-ELANE antibody (30-267) (STJ114982)

SKU:
STJ114982

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ELANE
Gene ID: 1991
Uniprot ID: ELNE_HUMAN
Immunogen Region: 30-267
Specificity: A synthetic peptide corresponding to a sequence within amino acids 30-267 of human Neutrophil Elastase (ELANE) (NP_001963.1).
Immunogen Sequence: IVGGRRARPHAWPFMVSLQL RGGHFCGATLIAPNFVMSAA HCVANVNVRAVRVVLGAHNL SRREPTRQVFAVQRIFENGY DPVNLLNDIVILQLNGSATI NANVQVAQLPAQGRRLGNGV QCLAMGWGLLGRNRGIASVL QELNVTVVTSLCRRSNVCTL VRGRQAGVCFGDSGSPLVCN GLIHGIASFVRGGCASGLYP DAFAPVAQFVNWIDSIIQRS EDNPCPHPRDPDPASRTH
Tissue Specificity Bone marrow cells. Neutrophil.
Function Serine protease that modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis. Promotes cleavage of GSDMB, thereby inhibiting pyroptosis. Promotes blood coagulation. Through the activation of the platelet fibrinogen receptor integrin alpha-IIb/beta-3, potentiates platelet aggregation induced by a threshold concentration of cathepsin G (CTSG). Cleaves and thus inactivates tissue factor pathway inhibitor (TFPI). Capable of killing E.coli but not S.aureus in vitro.digests outer membrane protein A (ompA) in E.coli and K.pneumoniae.
Protein Name Neutrophil Elastase
Bone Marrow Serine Protease
Elastase-2
Human Leukocyte Elastase
Hle
Medullasin
Pmn Elastase
Database Links Reactome: R-HSA-1442490
Reactome: R-HSA-1474228
Reactome: R-HSA-1592389
Reactome: R-HSA-5620971
Reactome: R-HSA-6798695
Reactome: R-HSA-6803157
Reactome: R-HSA-977606
Cellular Localisation Cytoplasmic Vesicle
Phagosome
Localized In Phagolysosomes Following Ingestion Of E
Coli By Neutrophils
Alternative Antibody Names Anti-Neutrophil Elastase antibody
Anti-Bone Marrow Serine Protease antibody
Anti-Elastase-2 antibody
Anti-Human Leukocyte Elastase antibody
Anti-Hle antibody
Anti-Medullasin antibody
Anti-Pmn Elastase antibody
Anti-ELANE antibody
Anti-ELA2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance