| Host: |
Rabbit |
| Applications: |
IF |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-EIF5B (901-1220) is suitable for use in Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
IF 1:10-1:100 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
EIF5B |
| Gene ID: |
9669 |
| Uniprot ID: |
IF2P_HUMAN |
| Immunogen Region: |
901-1220 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 901-1220 of human EIF5B (NP_056988.3). |
| Immunogen Sequence: |
IRGLLLPPPMKELRVKNQYE KHKEVEAAQGVKILGKDLEK TLAGLPLLVAYKEDEIPVLK DELIHELKQTLNAIKLEEKG VYVQASTLGSLEALLEFLKT SEVPYAGINIGPVHKKDVMK ASVMLEHDPQYAVILAFDVR IERDAQEMADSLGVRIFSAE IIYHLFDAFTKYRQDYKKQK QEEFKHIAVFPCKIKILPQY IFNSRDPIVMGVTVEAGQVK QGTPMCVPSKNFVDIGIVT |
| Post Translational Modifications | (Microbial infection) Cleaved and inactivated by the protease 3C of poliovirus, Coxsackievirus B3 and Human rhinovirus 14, allowing the virus to shutoff the host cell translation. |
| Function | Plays a role in translation initiation. Ribosome-dependent GTPase that promotes the joining of the 60S ribosomal subunit to the pre-initiation complex to form the 80S initiation complex with the initiator methionine-tRNA in the P-site base paired to the start codon. Together with eIF1A (EIF1AX), actively orients the initiator methionine-tRNA in a conformation that allows 60S ribosomal subunit joining to form the 80S initiation complex. Is released after formation of the 80S initiation complex. Its GTPase activity is not essential for ribosomal subunits joining, but GTP hydrolysis is needed for eIF1A (EIF1AX) ejection quickly followed by EIF5B release to form elongation-competent ribosomes. In contrast to its procaryotic homolog, does not promote recruitment of Met-rRNA to the small ribosomal subunit. |
| Protein Name | Eukaryotic Translation Initiation Factor 5bEif-5bTranslation Initiation Factor If-2 |
| Database Links | Reactome: R-HSA-72706 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Eukaryotic Translation Initiation Factor 5b antibodyAnti-Eif-5b antibodyAnti-Translation Initiation Factor If-2 antibodyAnti-EIF5B antibodyAnti-IF2 antibodyAnti-KIAA0741 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
