| Host: | Rabbit |
| Applications: | WB/IHC-P/ELISA |
| Reactivity: | Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: | Monoclonal |
| Clone ID: | SRM |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 IHC-P:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Gene Symbol: | EIF4G1 |
| Gene ID: | 1981 |
| Uniprot ID: | IF4G1_HUMAN |
| Immunogen Region: | 551-650 aa |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 551-650 of human EIF4G1 (NP_937884.2). |
| Immunogen Sequence: | GSNPGPESEGSGVPPRPEEA DETWDSKEDKIHNAENIQPG EQKYEYKSDQWKPLNLEEKK RYDREFLLGFQFIFASMQKP EGLPHISDVVLDKANKTPLR |
| Post Translational Modifications | Phosphorylated at multiple sites in vivo. Phosphorylation at Ser-1185 by PRKCA induces binding to MKNK1. Following infection by certain enteroviruses, rhinoviruses and aphthoviruses, EIF4G1 is cleaved by the viral protease 2A, or the leader protease in the case of aphthoviruses. This shuts down the capped cellular mRNA transcription. |
| Function | Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. Exists in two complexes, either with EIF1 or with EIF4E (mutually exclusive). Together with EIF1, is required for leaky scanning, in particular for avoiding cap-proximal start codon. Together with EIF4E, antagonizes the scanning promoted by EIF1-EIF4G1 and locates the start codon (through a TISU element) without scanning. As a member of the eIF4F complex, required for endoplasmic reticulum stress-induced ATF4 mRNA translation. |
| Protein Name | Eukaryotic Translation Initiation Factor 4 Gamma 1 Eif-4-Gamma 1 Eif-4g 1 Eif-4g1 P220 |
| Database Links | Reactome: R-HSA-1169408 Reactome: R-HSA-156827 Reactome: R-HSA-166208 Reactome: R-HSA-429947 Reactome: R-HSA-450408 Reactome: R-HSA-72649 Reactome: R-HSA-72662 Reactome: R-HSA-72702 Reactome: R-HSA-72706 Reactome: R-HSA-9010553 Reactome: R-HSA-975956 Reactome: R-HSA-975957 Reactome: R-HSA-9820841 Reactome: R-HSA-9820865 |
| Cellular Localisation | Cytoplasm Nucleus Stress Granule |
| Alternative Antibody Names | Anti-Eukaryotic Translation Initiation Factor 4 Gamma 1 antibody Anti-Eif-4-Gamma 1 antibody Anti-Eif-4g 1 antibody Anti-Eif-4g1 antibody Anti-P220 antibody Anti-EIF4G1 antibody Anti-EIF4F antibody Anti-EIF4G antibody Anti-EIF4GI antibody |
Information sourced from Uniprot.org
