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Anti-EIF4G1 antibody (400-500) (STJ29690)

Western blot analysis of extracts of HeLa cells, using EIF4G1 antibody (STJ29690) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using EIF4G1 rabbit polyclonal antibody (STJ29690) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
Immunoprecipitation analysis of 300 Mu g extracts of Hela cells using 3 Mu g EIF4G1 antibody (STJ29690). Western blot was performed from the immunoprecipitate using EIF4G1 antibody (STJ29690) at a dilution of 1:500.

Anti-EIF4G1 antibody (400-500) (STJ29690)

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STJ29690

£112.50 - £522.50

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General Information
Product Properties
Target Information
Additional Info
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Host:	Rabbit
Applications:	WB/IHC/IP
Reactivity:	Human
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-EIF4G1 (400-500) is suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification:	Affinity purification
Dilution Range:	WB 1:100-1:500 IHC-P 1:50-1:200 IP 1:100-1:500
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	EIF4G1
Gene ID:	1981
Uniprot ID:	IF4G1_HUMAN
Immunogen Region:	400-500
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 400-500 of human EIF4G1 (NP_886553.3).
Immunogen Sequence:	PEELLNGAPSPPAVDLSPVS EPEEQAKEVTASMAPPTIPS ATPATAPSATSPAQEEEMEE EEEEEEGEAGEAGEAESEKG GEELLPPESTPIPANLSQNL E

Post Translational Modifications	Phosphorylated at multiple sites in vivo. Phosphorylation at Ser-1185 by PRKCA induces binding to MKNK1. Following infection by certain enteroviruses, rhinoviruses and aphthoviruses, EIF4G1 is cleaved by the viral protease 2A, or the leader protease in the case of aphthoviruses. This shuts down the capped cellular mRNA transcription.
Function	Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. Exists in two complexes, either with EIF1 or with EIF4E (mutually exclusive). Together with EIF1, is required for leaky scanning, in particular for avoiding cap-proximal start codon. Together with EIF4E, antagonizes the scanning promoted by EIF1-EIF4G1 and locates the start codon (through a TISU element) without scanning. As a member of the eIF4F complex, required for endoplasmic reticulum stress-induced ATF4 mRNA translation.
Protein Name	Eukaryotic Translation Initiation Factor 4 Gamma 1 Eif-4-Gamma 1 Eif-4g 1 Eif-4g1 P220
Database Links	Reactome: R-HSA-1169408 Reactome: R-HSA-156827 Reactome: R-HSA-166208 Reactome: R-HSA-429947 Reactome: R-HSA-450408 Reactome: R-HSA-72649 Reactome: R-HSA-72662 Reactome: R-HSA-72702 Reactome: R-HSA-72706 Reactome: R-HSA-9010553 Reactome: R-HSA-975956 Reactome: R-HSA-975957
Cellular Localisation	Cytoplasm Nucleus Stress Granule
Alternative Antibody Names	Anti-Eukaryotic Translation Initiation Factor 4 Gamma 1 antibody Anti-Eif-4-Gamma 1 antibody Anti-Eif-4g 1 antibody Anti-Eif-4g1 antibody Anti-P220 antibody Anti-EIF4G1 antibody Anti-EIF4F antibody Anti-EIF4G antibody Anti-EIF4GI antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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