| Short Description : | Rabbit polyclonal EIF4B (352-611) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IF/ICC/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | EIF4B |
| Gene ID: | 1975 |
| Uniprot ID: | IF4B_HUMAN |
| Immunogen Region: | 352-611 |
| Immunogen Sequence: | SQSTRAASIFGGAKPVDTAA REREVEERLQKEQEKLQRQL DEPKLERRPRERHPSWRSEE TQERERSRTGSESSQTGTST TSSRNARRRESEKSLENETL NKEEDCHSPTSKPPKPDQPL KVMPAPPPKENAWVKRSSNP PARSQSSDTEQQSPTSGGGK VAPAQPSEEGPGRKDENKVD GMNAPKGQTGNSSRGPGDGG NRDHWKESDRKDGKKDQDSR SAPEPKKPEENPASKFSSA |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 352-611 of human EIF4B (NP_001408.2). |
| Post Translational Modifications | Phosphorylated at Ser-422 by RPS6KA1 and RPS6KB1.phosphorylation enhances the affinity of EIF4B for the EIF3 complex. In response to mTORC1 activation, RPS6KA1-mediated phosphorylation at 'Ser-406' and 'Ser-422' stimulates bicarbonate cotransporter SLC4A7 mRNA translation, increasing SLC4A7 protein abundance and function. |
| Function | Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F. |
| Protein Name | Eukaryotic Translation Initiation Factor 4bEif-4b |
| Database Links | Reactome: R-HSA-156827Reactome: R-HSA-166208Reactome: R-HSA-429947Reactome: R-HSA-72649Reactome: R-HSA-72662Reactome: R-HSA-72702Reactome: R-HSA-72706Reactome: R-HSA-9820841Reactome: R-HSA-9820865 |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-Eukaryotic Translation Initiation Factor 4b antibodyAnti-Eif-4b antibodyAnti-EIF4B antibody |
Information sourced from Uniprot.org
