| Host: | Rabbit |
| Applications: | WB |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Eukaryotic initiation factor 4A-III (218-268 aa) is suitable for use in Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-2000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | EIF4A3 |
| Gene ID: | 9775 |
| Uniprot ID: | IF4A3_HUMAN |
| Immunogen Region: | 218-268 aa |
| Specificity: | This antibody detects endogenous levels of IF4A3 at Human/Mouse/Rat |
| Immunogen: | Synthesized peptide derived from the human IF4A3 at the amino acid range 218-268 |
| Function | ATP-dependent RNA helicase. Involved in pre-mRNA splicing as component of the spliceosome. Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH-RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The inhibition of ATPase activity by the MAGOH-RBM8A heterodimer increases the RNA-binding affinity of the EJC. Involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Shows higher affinity for single-stranded RNA in an ATP-bound core EJC complex than after the ATP is hydrolyzed. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes).specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S).the function is different from the established EJC assembly. Involved in craniofacial development. |
| Protein Name | Eukaryotic Initiation Factor 4a-Iii Eif-4a-Iii Eif4a-Iii Atp-Dependent Rna Helicase Ddx48 Atp-Dependent Rna Helicase Eif4a-3 Dead Box Protein 48 Eukaryotic Initiation Factor 4a-Like Nuk-34 Eukaryotic Translation Initiation Factor 4a Isoform 3 Nuclear Matrix Protein 265 Nmp 265 Hnmp 265 Cleaved Into - Eukaryotic Initiation Factor 4a-Iii - N-Terminally Processed |
| Database Links | Reactome: R-HSA-1169408 Reactome: R-HSA-159236 Reactome: R-HSA-429947 Reactome: R-HSA-72163 Reactome: R-HSA-72187 Reactome: R-HSA-73856 Reactome: R-HSA-9010553 Reactome: R-HSA-975957 Reactome: R-HSA-9820841 Reactome: R-HSA-9820865 |
| Cellular Localisation | Nucleus Nucleus Speckle Cytoplasm Nucleocytoplasmic Shuttling Protein Travels To The Cytoplasm As Part Of The Exon Junction Complex (Ejc) Bound To Mrna Detected In Dendritic Layer As Well As The Nuclear And Cytoplasmic (Somatic) Compartments Of Neurons Colocalizes With Stau1 And Fmr1 In Dendrites |
| Alternative Antibody Names | Anti-Eukaryotic Initiation Factor 4a-Iii antibody Anti-Eif-4a-Iii antibody Anti-Eif4a-Iii antibody Anti-Atp-Dependent Rna Helicase Ddx48 antibody Anti-Atp-Dependent Rna Helicase Eif4a-3 antibody Anti-Dead Box Protein 48 antibody Anti-Eukaryotic Initiation Factor 4a-Like Nuk-34 antibody Anti-Eukaryotic Translation Initiation Factor 4a Isoform 3 antibody Anti-Nuclear Matrix Protein 265 antibody Anti-Nmp 265 antibody Anti-Hnmp 265 Cleaved Into - Eukaryotic Initiation Factor 4a-Iii - N-Terminally Processed antibody Anti-EIF4A3 antibody Anti-DDX48 antibody Anti-KIAA0111 antibody |
Information sourced from Uniprot.org