| Host: | Rabbit |
| Applications: | WB/IHC-P/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: | Monoclonal |
| Clone ID: | S9MR |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | AGO3 |
| Gene ID: | 192669 |
| Uniprot ID: | AGO3_HUMAN |
| Immunogen Region: | 721-860 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 721-860 of human eIF2C3 (Q9H9G7). |
| Immunogen Sequence: | RTERVGRSGNIPAGTTVDTD ITHPYEFDFYLCSHAGIQGT SRPSHYHVLWDDNCFTADEL QLLTYQLCHTYVRCTRSVSI PAPAYYAHLVAFRARYHLVD KEHDSAEGSHVSGQSNGRDP QALAKAVQIHQDTLRTMYFA |
| Post Translational Modifications | Ubiquitinated on surface-exposed lysines by a SCF-like E3 ubiquitin-protein ligase complex containing ZSWIM8 during target-directed microRNA degradation (TDMD), a process that mediates degradation of microRNAs (miRNAs). Ubiquitination by the SCF-like E3 ubiquitin-protein ligase complex containing ZSWIM8 leads to its subsequent degradation, thereby exposing miRNAs for degradation. ZSWIM8 recognizes and binds AGO3 when it is engaged with a TDMD target. |
| Function | Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Proposed to be involved in stabilization of small RNA derivates (siRNA) derived from processed RNA polymerase III-transcribed Alu repeats containing a DR2 retinoic acid response element (RARE) in stem cells and in the subsequent siRNA-dependent degradation of a subset of RNA polymerase II-transcribed coding mRNAs by recruiting a mRNA decapping complex involving EDC4. Possesses RNA slicer activity but only on select RNAs bearing 5'- and 3'-flanking sequences to the region of guide-target complementarity. |
| Protein Name | Protein Argonaute-3Argonaute3Hago3Argonaute Risc Catalytic Component 3Eukaryotic Translation Initiation Factor 2c 3Eif-2c 3Eif2c 3 |
| Database Links | Reactome: R-HSA-1912408Reactome: R-HSA-203927Reactome: R-HSA-2559580Reactome: R-HSA-2559585Reactome: R-HSA-4086398Reactome: R-HSA-426486Reactome: R-HSA-426496Reactome: R-HSA-5628897Reactome: R-HSA-5687128Reactome: R-HSA-8853884Reactome: R-HSA-8934593Reactome: R-HSA-8936459Reactome: R-HSA-8943723Reactome: R-HSA-8948700Reactome: R-HSA-8986944Reactome: R-HSA-9018519Reactome: R-HSA-9022692Reactome: R-HSA-9029569Reactome: R-HSA-9725371Reactome: R-HSA-9759811Reactome: R-HSA-9768778Reactome: R-HSA-9824594Reactome: R-HSA-9839394 |
| Cellular Localisation | CytoplasmP-Body |
| Alternative Antibody Names | Anti-Protein Argonaute-3 antibodyAnti-Argonaute3 antibodyAnti-Hago3 antibodyAnti-Argonaute Risc Catalytic Component 3 antibodyAnti-Eukaryotic Translation Initiation Factor 2c 3 antibodyAnti-Eif-2c 3 antibodyAnti-Eif2c 3 antibodyAnti-AGO3 antibodyAnti-EIF2C3 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
