| Host: | Goat |
| Applications: | Pep-ELISA/WB-Trf/IHC |
| Reactivity: | Human/Mouse/Rat/Dog |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-EIF2C1/AGO1 (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:16000.WB: In transfected HEK293 transiently expressing Human EIF2C1bands of approx. 110kDa and 35kDa are observed. The 110kDa band is not observed in the non-transfected HEK293. The calculated m |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | AGO1 |
| Gene ID: | 26523 |
| Uniprot ID: | AGO1_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_036331.1 |
| Specificity: | This product is not expected to cross-react with EIF2C2, EIF2C3 and EIF2C4. |
| Immunogen Sequence: | KNASYNLDPYIQEF |
| Function | Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs). |
| Protein Name | Protein Argonaute-1Argonaute1Hago1Argonaute Risc Catalytic Component 1Eukaryotic Translation Initiation Factor 2c 1Eif-2c 1Eif2c 1Putative Rna-Binding Protein Q99 |
| Database Links | Reactome: R-HSA-1912408Reactome: R-HSA-203927Reactome: R-HSA-2559580Reactome: R-HSA-2559585Reactome: R-HSA-4086398Reactome: R-HSA-426486Reactome: R-HSA-426496Reactome: R-HSA-5578749Reactome: R-HSA-5628897Reactome: R-HSA-5687128Reactome: R-HSA-8853884Reactome: R-HSA-8934593Reactome: R-HSA-8936459Reactome: R-HSA-8943723Reactome: R-HSA-8948700Reactome: R-HSA-8986944Reactome: R-HSA-9018519Reactome: R-HSA-9022692Reactome: R-HSA-9029569Reactome: R-HSA-9725371Reactome: R-HSA-9759811Reactome: R-HSA-9768778Reactome: R-HSA-9824594Reactome: R-HSA-9839394 |
| Cellular Localisation | CytoplasmP-Body |
| Alternative Antibody Names | Anti-Protein Argonaute-1 antibodyAnti-Argonaute1 antibodyAnti-Hago1 antibodyAnti-Argonaute Risc Catalytic Component 1 antibodyAnti-Eukaryotic Translation Initiation Factor 2c 1 antibodyAnti-Eif-2c 1 antibodyAnti-Eif2c 1 antibodyAnti-Putative Rna-Binding Protein Q99 antibodyAnti-AGO1 antibodyAnti-EIF2C1 antibody |
Information sourced from Uniprot.org