Host: | Goat |
Applications: | Pep-ELISA/WB-Trf/IHC |
Reactivity: | Human/Mouse/Rat/Dog |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: | Goat polyclonal antibody anti-EIF2C1/AGO1 (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
Clonality: | Polyclonal |
Conjugation: | Unconjugated |
Isotype: | IgG |
Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Concentration: | 0.5 mg/mL |
Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:16000. WB: In transfected HEK293 transiently expressing Human EIF2C1bands of approx. 110kDa and 35kDa are observed. The 110kDa band is not observed in the non-transfected HEK293. The calculated m |
Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Gene Symbol: | AGO1 |
Gene ID: | 26523 |
Uniprot ID: | AGO1_HUMAN |
Immunogen Region: | Internal |
Accession Number: | NP_036331.1 |
Specificity: | This product is not expected to cross-react with EIF2C2, EIF2C3 and EIF2C4. |
Immunogen Sequence: | KNASYNLDPYIQEF |
Function | Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs). |
Protein Name | Protein Argonaute-1 Argonaute1 Hago1 Argonaute Risc Catalytic Component 1 Eukaryotic Translation Initiation Factor 2c 1 Eif-2c 1 Eif2c 1 Putative Rna-Binding Protein Q99 |
Database Links | Reactome: R-HSA-1912408 Reactome: R-HSA-203927 Reactome: R-HSA-2559580 Reactome: R-HSA-2559585 Reactome: R-HSA-4086398 Reactome: R-HSA-426486 Reactome: R-HSA-426496 Reactome: R-HSA-5578749 Reactome: R-HSA-5628897 Reactome: R-HSA-5687128 Reactome: R-HSA-8853884 Reactome: R-HSA-8934593 Reactome: R-HSA-8936459 Reactome: R-HSA-8943723 Reactome: R-HSA-8948700 Reactome: R-HSA-8986944 Reactome: R-HSA-9018519 Reactome: R-HSA-9022692 Reactome: R-HSA-9029569 Reactome: R-HSA-9725371 Reactome: R-HSA-9759811 Reactome: R-HSA-9768778 Reactome: R-HSA-9824594 Reactome: R-HSA-9839394 |
Cellular Localisation | Cytoplasm P-Body |
Alternative Antibody Names | Anti-Protein Argonaute-1 antibody Anti-Argonaute1 antibody Anti-Hago1 antibody Anti-Argonaute Risc Catalytic Component 1 antibody Anti-Eukaryotic Translation Initiation Factor 2c 1 antibody Anti-Eif-2c 1 antibody Anti-Eif2c 1 antibody Anti-Putative Rna-Binding Protein Q99 antibody Anti-AGO1 antibody Anti-EIF2C1 antibody |
Information sourced from Uniprot.org