Immunoprecipitation analysis of 200 Mu g extracts of A-549 cells using 3 Mu g eIF2B epsilon (eIF2B epsilon (EIF2B5) ) antibody ( STJ112301). Western blot was performed from the immunoprecipitate using eIF2B epsilon (eIF2B epsilon (EIF2B5) ) antibody ( STJ112301) at a dilution of 1:1000.

Anti-EIF2B5 antibody (442-721) (STJ112301)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ112301
๐Ÿšš Free UK Delivery on orders over ยฃ150
Processing The item has been added
Enquire For Bulk Order
✓ 1-Year Performance Guarantee | SDS & Datasheet Included | Expert Tech Support | Covered by St John's Laboratory Guarantee

General Information

Short DescriptionRabbit polyclonal EIF2B5 (442-721) antibody for use in WB, IF, ICC, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IF/ICC/IP/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
IF/ICC:1:50-1:200
IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements
FormulationPBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolEIF2B5
Gene ID8893
Uniprot IDEI2BE_HUMAN
Immunogen Region442-721
Immunogen SequenceTLPEGSVISLHPPDAEEDED DGEFSDDSGADQEKDKVKMK GYNPAEVGAAGKGYLWKAAG MNMEEEEELQQNLWGLKINM EEESESESEQSMDSEEPDSR GGSPQMDDIKVFQNEVLGTL QRGKEENISCDNLVLEINSL KYAYNISLKEVMQVLSHVVL EFPLQQMDSPLDSSRYCALL LPLLKAWSPVFRNYIKRAAD HLEALAAIEDFFLEHEALGI SMAKVLMAFYQLEILAEET
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 442-721 of human eIF2B epsilon (eIF2B epsilon (EIF2B5) ) (NP_003898.2).

Additional Info

Post Translational Modifications Phosphorylated at Ser-544 by DYRK2.this is required for subsequent phosphorylation by GSK3B. Phosphorylated on serine and threonine residues by GSK3B.phosphorylation inhibits its function. Polyubiquitinated, probably by NEDD4.
Function Acts as a component of the translation initiation factor 2B (eIF2B) complex, which catalyzes the exchange of GDP for GTP on eukaryotic initiation factor 2 (eIF2) gamma subunit. Its guanine nucleotide exchange factor activity is repressed when bound to eIF2 complex phosphorylated on the alpha subunit, thereby limiting the amount of methionyl-initiator methionine tRNA available to the ribosome and consequently global translation is repressed.
Protein Name Translation Initiation Factor Eif2b Subunit Epsilon
Eif2b Gdp-Gtp Exchange Factor Subunit Epsilon
Database Links Reactome: R-HSA-72731
Cellular Localisation Cytoplasm
Cytosol
Alternative Antibody Names Anti-Translation Initiation Factor Eif2b Subunit Epsilon antibody
Anti-Eif2b Gdp-Gtp Exchange Factor Subunit Epsilon antibody
Anti-EIF2B5 antibody
Anti-EIF2BE antibody

Information sourced from Uniprot.org

Citations

Product Review

!