• Western blot analysis of extracts of various cell lines, using EIF2B3 antibody (STJ112300) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-EIF2B3 antibody (1-270) (STJ112300)

SKU:
STJ112300

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EIF2B3
Gene ID: 8891
Uniprot ID: EI2BG_HUMAN
Immunogen Region: 1-270
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human EIF2B3 (NP_065098.1).
Immunogen Sequence: MEFQAVVMAVGGGSRMTDLT SSIPKPLLPVGNKPLIWYPL NLLERVGFEEVIVVTTRDVQ KALCAEFKMKMKPDIVCIPD DADMGTADSLRYIYPKLKTD VLVLSCDLITDVALHEVVDL FRAYDASLAMLMRKGQDSIE PVPGQKGKKKAVEQRDFIGV DSTGKRLLFMANEADLDEEL VIKGSILQKHPRIRFHTGLV DAHLYCLKKYIVDFLMENGS ITSIRSELIPYLVRKQFSS
Function Acts as a component of the translation initiation factor 2B (eIF2B) complex, which catalyzes the exchange of GDP for GTP on the eukaryotic initiation factor 2 (eIF2) complex gamma subunit. Its guanine nucleotide exchange factor activity is repressed when bound to eIF2 complex phosphorylated on the alpha subunit, thereby limiting the amount of methionyl-initiator methionine tRNA available to the ribosome and consequently global translation is repressed.
Protein Name Translation Initiation Factor Eif2b Subunit Gamma
Eif2b Gdp-Gtp Exchange Factor Subunit Gamma
Database Links Reactome: R-HSA-72731
Cellular Localisation Cytoplasm
Cytosol
Alternative Antibody Names Anti-Translation Initiation Factor Eif2b Subunit Gamma antibody
Anti-Eif2b Gdp-Gtp Exchange Factor Subunit Gamma antibody
Anti-EIF2B3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance