| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-EIF2AK1 (371-630) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
EIF2AK1 |
| Gene ID: |
27102 |
| Uniprot ID: |
E2AK1_HUMAN |
| Immunogen Region: |
371-630 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 371-630 of human EIF2AK1 (NP_055228.2). |
| Immunogen Sequence: |
QTEAQYHLMLHIQMQLCELS LWDWIVERNKRGREYVDESA CPYVMANVATKIFQELVEGV FYIHNMGIVHRDLKPRNIFL HGPDQQVKIGDFGLACTDIL QKNTDWTNRNGKRTPTHTSR VGTCLYASPEQLEGSEYDAK SDMYSLGVVLLELFQPFGTE MERAEVLTGLRTGQLPESLR KRCPVQAKYIQHLTRRNSSQ RPSAIQLLQSELFQNSGNVN LTLQMKIIEQEKEIAELKK |
| Post Translational Modifications | Activated by autophosphorylation.phosphorylated predominantly on serine and threonine residues, but also on tyrosine residues. Autophosphorylation at Thr-488 is required for kinase activation. The active autophosphorylated form apparently is largely refractory to cellular heme fluctuations. |
| Function | Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) in response to various stress conditions. Key activator of the integrated stress response (ISR) required for adaptation to various stress, such as heme deficiency, oxidative stress, osmotic shock, mitochondrial dysfunction and heat shock. EIF2S1/eIF-2-alpha phosphorylation in response to stress converts EIF2S1/eIF-2-alpha in a global protein synthesis inhibitor, leading to a global attenuation of cap-dependent translation, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming. Acts as a key sensor of heme-deficiency: in normal conditions, binds hemin via a cysteine thiolate and histidine nitrogenous coordination, leading to inhibit the protein kinase activity. This binding occurs with moderate affinity, allowing it to sense the heme concentration within the cell: heme depletion relieves inhibition and stimulates kinase activity, activating the ISR. Thanks to this unique heme-sensing capacity, plays a crucial role to shut off protein synthesis during acute heme-deficient conditions. In red blood cells (RBCs), controls hemoglobin synthesis ensuring a coordinated regulation of the synthesis of its heme and globin moieties. It thereby plays an essential protective role for RBC survival in anemias of iron deficiency. Iron deficiency also triggers activation by full-length DELE1. Also activates the ISR in response to mitochondrial dysfunction: HRI/EIF2AK1 protein kinase activity is activated upon binding to the processed form of DELE1 (S-DELE1), thereby promoting the ATF4-mediated reprogramming. |
| Protein Name | Eukaryotic Translation Initiation Factor 2-Alpha Kinase 1Heme-Controlled RepressorHcrHeme-Regulated Eukaryotic Initiation Factor Eif-2-Alpha KinaseHeme-Regulated InhibitorHhriHemin-Sensitive Initiation Factor 2-Alpha Kinase |
| Database Links | Reactome: R-HSA-9648895 |
| Alternative Antibody Names | Anti-Eukaryotic Translation Initiation Factor 2-Alpha Kinase 1 antibodyAnti-Heme-Controlled Repressor antibodyAnti-Hcr antibodyAnti-Heme-Regulated Eukaryotic Initiation Factor Eif-2-Alpha Kinase antibodyAnti-Heme-Regulated Inhibitor antibodyAnti-Hhri antibodyAnti-Hemin-Sensitive Initiation Factor 2-Alpha Kinase antibodyAnti-EIF2AK1 antibodyAnti-HRI antibodyAnti-KIAA1369 antibodyAnti-PRO1362 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
