Anti-EIF2AK1 antibody (337-630) (STJ116054)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal EIF2AK1 (337-630) antibody for use in WB, IHC-P and ELISA in human and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:100-1:500ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | EIF2AK1 |
| Gene ID | 27102 |
| Uniprot ID | E2AK1_HUMAN |
| Immunogen Region | 337-630 |
| Immunogen Sequence | SIVEQQLPLRRNSHLEESFT STEESSEENVNFLGQTEAQY HLMLHIQMQLCELSLWDWIV ERNKRGREYVDESACPYVMA NVATKIFQELVEGVFYIHNM GIVHRDLKPRNIFLHGPDQQ VKIGDFGLACTDILQKNTDW TNRNGKRTPTHTSRVGTCLY ASPEQLEGSEYDAKSDMYSL GVVLLELFQPFGTEMERAEV LTGLRTGQLPESLRKRCPVQ AKYIQHLTRRNSSQRPSAI |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 337-586 of human EIF2AK1 (NP_055228.2). |
Additional Info
| Post Translational Modifications | Activated by autophosphorylation.phosphorylated predominantly on serine and threonine residues, but also on tyrosine residues. Autophosphorylation at Thr-488 is required for kinase activation. The active autophosphorylated form apparently is largely refractory to cellular heme fluctuations. Ubiquitinated and degraded by the SIFI complex once the mitochondrial stress has been resolved, thereby providing stress response silencing. Within the SIFI complex, UBR4 initiates ubiquitin chain that are further elongated or branched by KCMF1. |
| Function | Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) in response to various stress conditions. Key activator of the integrated stress response (ISR) required for adaptation to various stress, such as heme deficiency, oxidative stress, osmotic shock, mitochondrial dysfunction and heat shock. EIF2S1/eIF-2-alpha phosphorylation in response to stress converts EIF2S1/eIF-2-alpha in a global protein synthesis inhibitor, leading to a global attenuation of cap-dependent translation, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming. Acts as a key sensor of heme-deficiency: in normal conditions, binds hemin via a cysteine thiolate and histidine nitrogenous coordination, leading to inhibit the protein kinase activity. This binding occurs with moderate affinity, allowing it to sense the heme concentration within the cell: heme depletion relieves inhibition and stimulates kinase activity, activating the ISR. Thanks to this unique heme-sensing capacity, plays a crucial role to shut off protein synthesis during acute heme-deficient conditions. In red blood cells (RBCs), controls hemoglobin synthesis ensuring a coordinated regulation of the synthesis of its heme and globin moieties. It thereby plays an essential protective role for RBC survival in anemias of iron deficiency. Iron deficiency also triggers activation by full-length DELE1. Also activates the ISR in response to mitochondrial dysfunction: HRI/EIF2AK1 protein kinase activity is activated upon binding to the processed form of DELE1 (S-DELE1), thereby promoting the ATF4-mediated reprogramming. Also acts as an activator of mitophagy in response to mitochondrial damage: catalyzes phosphorylation of eIF-2-alpha (EIF2S1) following activation by S-DELE1, thereby promoting mitochondrial localization of EIF2S1, triggering PRKN-independent mitophagy. |
| Protein Name | Eukaryotic Translation Initiation Factor 2-Alpha Kinase 1Heme-Controlled RepressorHcrHeme-Regulated Eukaryotic Initiation Factor Eif-2-Alpha KinaseHeme-Regulated InhibitorHhriHemin-Sensitive Initiation Factor 2-Alpha Kinase |
| Database Links | Reactome: R-HSA-9648895Reactome: R-HSA-9840373 |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-Eukaryotic Translation Initiation Factor 2-Alpha Kinase 1 antibodyAnti-Heme-Controlled Repressor antibodyAnti-Hcr antibodyAnti-Heme-Regulated Eukaryotic Initiation Factor Eif-2-Alpha Kinase antibodyAnti-Heme-Regulated Inhibitor antibodyAnti-Hhri antibodyAnti-Hemin-Sensitive Initiation Factor 2-Alpha Kinase antibodyAnti-EIF2AK1 antibodyAnti-HRI antibodyAnti-KIAA1369 antibodyAnti-PRO1362 antibody |
Information sourced from Uniprot.org